Cttn Knockout cell line (B16-F10)

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Cttn Knockout cell line (B16-F10) Order

Cttn Knockout cell line (B16-F10)
Catalog#
YKO-M281
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Cttn Knockout cell line (B16-F10)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
Cttn Knockout cell line (B16-F10)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:4
Species
Mouse
Gene Name
Cttn
Gene ID
13043
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10%FBS
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
Target region
Knockout region: Exon 4 (based on transcript Cttn-202)
Validation: PCR+Sanger Sequencing
Cttn Knockout cell line (B16-F10) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:B16-F10		 STR Info (Cell bank)
Cell Line:B16-F10
Allele1Allele2Allele3Allele4Allele1Allele2Allele3
18-3 15.016.015.016.0
4-2 20.321.320.321.3
6-7 15.015.0
19-2 13.014.013.014.0
1-2 19.020.019.020.0
7-1 26.226.2
8-1 16.017.016.017.0
1-1 17.018.019.017.018.019.0
3-2 14.015.014.015.0
2-1 16.016.0
15-3 22.323.322.323.3
6-4 18.019.018.019.0
13-1 17.118.117.118.1
11-2 16.017.016.017.0
17-2 15.016.017.015.016.017.0
12-1 17.018.017.018.0
5-5 16.020.016.020.0
X-1 28.028.0
TH01
D5S818
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Cttn gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the B16-F10 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Cttn gene knockout Cttn Knockout cell line (B16-F10) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
Cttn Knockout cell line (B16-F10)
Cttn Knockout cell line (B16-F10)
Catalog#
YKO-M281
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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