ENSG00000280441 Knockout cell line (TC71)

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ENSG00000280441 Knockout cell line (TC71) Order

ENSG00000280441 Knockout cell line (TC71)
Catalog#
YKO-H1506
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
ENSG00000280441 Knockout cell line (TC71)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
ENSG00000280441 Knockout cell line (TC71)
Species
Human
Gene Name
ENSG00000280441
Gene ID
-
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ENSG00000280441 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the TC71 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ENSG00000280441 gene knockout ENSG00000280441 Knockout cell line (TC71) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
ENSG00000280441 Knockout cell line (TC71)
ENSG00000280441 Knockout cell line (TC71)
Catalog#
YKO-H1506
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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