ESR1 Knockout cell line (MDA-MB-231)

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ESR1 Knockout cell line (MDA-MB-231) Order

ESR1 Knockout cell line (MDA-MB-231)
Catalog#
YKO-H1573
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
ESR1 Knockout cell line (MDA-MB-231)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
ESR1 Knockout cell line (MDA-MB-231)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Gene Name
ESR1
Gene ID
2099
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%L-15+10%FBS(Recommend to use Ubigene-optimized culture medium)
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
Target region
Knockout region: Exon 2 (based on transcript ESR1-201)
Validation: PCR+Sanger Sequencing
ESR1 Knockout cell line (MDA-MB-231) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:MDA-MB-231		 STR Info (Cell bank)
Cell Line:MDA-MB-231
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12121212
D13S317 13131313
D7S820 8989
D16S539 12121212
VWA 15181518
TH01 79.379.3
AMEL XXXX
TPOX 8989
CSF1PO 12131213
D12S391 1718
FGA 2223
D2S1338 2021
D21S11 33.233.2
D18S51 1116
D8S1179 1313
D3S1358 1616
D6S1043 1818
PENTAE 1111
D19S433 1114
PENTAD 1114
D1S1656 1517
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ESR1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the MDA-MB-231 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ESR1 gene knockout ESR1 Knockout cell line (MDA-MB-231) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
ESR1 Knockout cell line (MDA-MB-231)
ESR1 Knockout cell line (MDA-MB-231)
Catalog#
YKO-H1573
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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