ESRRA Knockout cell line (OVCAR-3)

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ESRRA Knockout cell line (OVCAR-3) Order

ESRRA Knockout cell line (OVCAR-3)
Catalog#
YKO-H1521
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
ESRRA Knockout cell line (OVCAR-3)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
ESRRA Knockout cell line (OVCAR-3)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:3
Species
Human
Gene Name
ESRRA
Gene ID
2101
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
80%RPMI-1640+20% FBS+0.01mg/mL Insulin
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
Target region
Knockout region: Exon 2 (based on transcript ESRRA-201)
Validation: PCR+Sanger Sequencing
ESRRA Knockout cell line (OVCAR-3) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:OVCAR-3		 STR Info (Cell bank)
Cell Line:OVCAR-3
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 12121212
D7S820 10101010
D16S539 12121212
VWA 17171717
TH01 9999
AMEL XXXX
TPOX 8888
CSF1PO 11121112
D12S391 1922
FGA 2121
D2S1338 1721
D21S11 2931.2
D18S51 1313
D8S1179 1015
D3S1358 1718
D6S1043 1011
PENTAE 713
D19S433 16.216.2
PENTAD 1213
D1S1656 1217.3
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ESRRA gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the OVCAR-3 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ESRRA gene knockout ESRRA Knockout cell line (OVCAR-3) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
ESRRA Knockout cell line (OVCAR-3)
ESRRA Knockout cell line (OVCAR-3)
Catalog#
YKO-H1521
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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