MAP2K2 Knockout cell line (THP-1)

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MAP2K2 Knockout cell line (THP-1) Order

MAP2K2 Knockout cell line (THP-1)
Catalog#
YKO-HT0687
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
MAP2K2 Knockout cell line (THP-1)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
MAP2K2 Knockout cell line (THP-1)
Species
Human
Gene Name
MAP2K2
Gene ID
5605
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript MAP2K2-201)
Validation: PCR+Sanger Sequencing
MAP2K2 Knockout cell line (THP-1) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the MAP2K2 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the THP-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, MAP2K2 gene knockout MAP2K2 Knockout cell line (THP-1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
MAP2K2 Knockout cell line (THP-1)
MAP2K2 Knockout cell line (THP-1)
Catalog#
YKO-HT0687
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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  • MAP2K2 Knockout cell line (U-2 OS)

    MAP2K2 Knockout cell line (U-2 OS)

    Organism: Human
    Gene Name: MAP2K2
    Gene ID: 5605
    Size: 1*10^6
    Catalog#: YKO-HT13164
    Request a Quote More details
  • MAP2K2 Knockout cell line (Jurkat)

    MAP2K2 Knockout cell line (Jurkat)

    Organism: Human
    Gene Name: MAP2K2
    Gene ID: 5605
    Size: 1*10^6
    Catalog#: YKO-HT9846
    Request a Quote More details
  • MAP2K2 Knockout cell line (Hep G2)

    MAP2K2 Knockout cell line (Hep G2)

    Organism: Human
    Gene Name: MAP2K2
    Gene ID: 5605
    Size: 1*10^6
    Catalog#: YKO-HT2447
    Request a Quote More details

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