Malt1 Knockout cell line (66cl4)

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Malt1 Knockout cell line (66cl4) Order

Malt1 Knockout cell line (66cl4)
Catalog#
YKO-M280
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Malt1 Knockout cell line (66cl4)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
Malt1 Knockout cell line (66cl4)
Species
Mouse
Gene Name
Malt1
Gene ID
240354
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
Target region
Knockout region: Exon 2 (based on transcript Malt1-202)
Validation: PCR+Sanger Sequencing
Malt1 Knockout cell line (66cl4) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Malt1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the 66cl4 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Malt1 gene knockout Malt1 Knockout cell line (66cl4) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
Malt1 Knockout cell line (66cl4)
Malt1 Knockout cell line (66cl4)
Catalog#
YKO-M280
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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