Mzf1 Knockout cell line (LX-2)

Gene knockout cell lines are generated using CRISPR-Cas9 based technology to precisely disrupt target genes, enabling functional genomics research, disease modeling, and target validation.

MZF1 (myeloid zinc finger 1) encodes a zinc finger-containing transcription factor that binds sequence-specific DNA regions associated with RNA polymerase II-dependent transcription. MZF1 can regulate gene expression in both positive and negative directions and is capable of forming protein homodimers. Through its DNA-binding and transcriptional regulatory activities, MZF1 may influence cellular programs involved in proliferation, differentiation, and other processes controlled by gene expression. The protein is primarily localized to the nucleoplasm.

Human hepatic stellate cells are represented by LX-2, an immortalized cell line derived from human hepatic stellate cells isolated from normal adult liver tissue. The cells retain several characteristics of activated hepatic stellate cells and are classified as a human liver stromal cell model. LX-2 is used as an in vitro model for investigating hepatic stellate cell biology and liver fibrogenesis.

Comprehensive quality control includes STR authentication, sterility testing (bacteria and fungi), and mycoplasma screening to ensure purity and performance. Genotype is confirmed by two rounds of PCR and Sanger sequencing, and complete validation reports are provided for make-to-order products.