Mzf1 Knockout cell line (LX-2)

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Mzf1 Knockout cell line (LX-2) Order

Mzf1 Knockout cell line (LX-2)
Catalog#
YKO-H1538
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Mzf1 Knockout cell line (LX-2)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
Mzf1 Knockout cell line (LX-2)
Cell Morphology
Epithelial-like, adheren
Passage ratio
1:2-1:4
Species
Human
Gene Name
Mzf1
Gene ID
7593
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
98%DMEM+2%FBS
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
Target region
Knockout region: Exon 2 (based on transcript MZF1-201)
Validation: PCR+Sanger Sequencing
Mzf1 Knockout cell line (LX-2) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:LX-2		 STR Info (Cell bank)
Cell Line:LX-2
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 11131113
D7S820 11111111
D16S539 13131313
VWA 17171717
TH01 9.39.39.39.3
AMEL XYXY
TPOX 8889
CSF1PO 10121012
D12S391 1517
FGA 2126
D2S1338 1717
D21S11 2831
D18S51 1212
D8S1179 1313
D3S1358 1315
D6S1043 1112
PENTAE 521
D19S433 1315.2
PENTAD 121315.0
D1S1656 1212
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Mzf1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the LX-2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Mzf1 gene knockout Mzf1 Knockout cell line (LX-2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
Mzf1 Knockout cell line (LX-2)
Mzf1 Knockout cell line (LX-2)
Catalog#
YKO-H1538
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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