NHEJ1 Knockout cell line (Hep G2)

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NHEJ1 Knockout cell line (Hep G2) Order

NHEJ1 Knockout cell line (Hep G2)
Catalog#
YKO-HT2760
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
NHEJ1 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
NHEJ1 Knockout cell line (Hep G2)
Species
Human
Gene Name
NHEJ1
Gene ID
79840
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript NHEJ1-201)
Validation: PCR+Sanger Sequencing
NHEJ1 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the NHEJ1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, NHEJ1 gene knockout NHEJ1 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
NHEJ1 Knockout cell line (Hep G2)
NHEJ1 Knockout cell line (Hep G2)
Catalog#
YKO-HT2760
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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  • NHEJ1 Knockout cell line (U-2 OS)

    NHEJ1 Knockout cell line (U-2 OS)

    Organism: Human
    Gene Name: NHEJ1
    Gene ID: 79840
    Size: 1*10^6
    Catalog#: YKO-HT13112
    Request a Quote More details
  • NHEJ1 Knockout cell line (HeLa)

    NHEJ1 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: NHEJ1
    Gene ID: 79840
    Size: 1*10^6
    Catalog#: YKO-XN80252
    Request a Quote More details
  • NHEJ1 Knockout cell line (HCT 116)

    NHEJ1 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: NHEJ1
    Gene ID: 79840
    Size: 1*10^6
    Catalog#: YKO-XN80251
    Request a Quote More details

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