NHLRC3 Knockout cell line (Hep G2)

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NHLRC3 Knockout cell line (Hep G2) Order

NHLRC3 Knockout cell line (Hep G2)
Catalog#
YKO-HT7484
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
NHLRC3 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
NHLRC3 Knockout cell line (Hep G2)
Species
Human
Gene Name
NHLRC3
Gene ID
387921
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript NHLRC3-202)
Validation: PCR+Sanger Sequencing
NHLRC3 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the NHLRC3 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, NHLRC3 gene knockout NHLRC3 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
NHLRC3 Knockout cell line (Hep G2)
NHLRC3 Knockout cell line (Hep G2)
Catalog#
YKO-HT7484
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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  • NHLRC3 Knockout cell line (HEK293)

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  • NHLRC3 Knockout cell line (HeLa)

    NHLRC3 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: NHLRC3
    Gene ID: 387921
    Size: 1*10^6
    Catalog#: YKO-XN91101
    Request a Quote More details
  • NHLRC3 Knockout cell line (HCT 116)

    NHLRC3 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: NHLRC3
    Gene ID: 387921
    Size: 1*10^6
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  • NHLRC3 Knockout cell line (HEK293)

    NHLRC3 Knockout cell line (HEK293)

    Organism: Human
    Gene Name: NHLRC3
    Gene ID: 387921
    Size: 1*10^6
    Catalog#: YKO-XN91099
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