NSMCE2 Knockout cell line (JAR)

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NSMCE2 Knockout cell line (JAR) Order

NSMCE2 Knockout cell line (JAR)
Catalog#
YKO-HA50478
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
NSMCE2 Knockout cell line (JAR)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
NSMCE2 Knockout cell line (JAR)
Cell Morphology
Epithelial-like, adheren
Passage ratio
1:2-1:3
Species
Human
Gene Name
NSMCE2
Gene ID
286053
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10%FBS
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:JAR		 STR Info (Cell bank)
Cell Line:JAR
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 10111011
D13S317 11111111
D7S820 10111011
D16S539 910910
VWA 16181618
TH01 6767
AMEL XYXY
TPOX 811811
CSF1PO 710710
D12S391 1820
FGA 2222
D2S1338 2021
D21S11 2829
D18S51 1415
D8S1179 1416
D3S1358 1617
D6S1043 1417
PENTAE 812
D19S433 1213
PENTAD 914
D1S1656 1415
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the NSMCE2 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the JAR cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, NSMCE2 gene knockout NSMCE2 Knockout cell line (JAR) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
NSMCE2 Knockout cell line (JAR)
NSMCE2 Knockout cell line (JAR)
Catalog#
YKO-HA50478
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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  • NSMCE2 Knockout cell line (AC16)

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    Gene Name: NSMCE2
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    Request a Quote More details
  • NSMCE2 Knockout cell line (BEAS-2B)

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    Gene Name: NSMCE2
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    Size: 1*10^6
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