PARK7 Knockout cell line (Hep G2)

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PARK7 Knockout cell line (Hep G2) Order

PARK7 Knockout cell line (Hep G2)
Catalog#
YKO-HT2345
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
PARK7 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
PARK7 Knockout cell line (Hep G2)
Species
Human
Gene Name
PARK7
Gene ID
11315
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 3 (based on transcript PARK7-201)
Validation: PCR+Sanger Sequencing
PARK7 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PARK7 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PARK7 gene knockout PARK7 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
PARK7 Knockout cell line (Hep G2)
PARK7 Knockout cell line (Hep G2)
Catalog#
YKO-HT2345
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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  • PARK7 Knockout cell line (U-2 OS)

    PARK7 Knockout cell line (U-2 OS)

    Organism: Human
    Gene Name: PARK7
    Gene ID: 11315
    Size: 1*10^6
    Catalog#: YKO-HT12995
    Request a Quote More details
  • PARK7 Knockout cell line (U-251 MG)

    PARK7 Knockout cell line (U-251 MG)

    Organism: Human
    Gene Name: PARK7
    Gene ID: 11315
    Size: 1*10^6
    Catalog#: YKO-HT12719
    Request a Quote More details
  • PARK7 Knockout cell line (Jurkat)

    PARK7 Knockout cell line (Jurkat)

    Organism: Human
    Gene Name: PARK7
    Gene ID: 11315
    Size: 1*10^6
    Catalog#: YKO-HT10815
    Request a Quote More details

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