PHKA1 Knockout cell line (NCI-H82)

Ubigene gene knockout cell lines mediated by optimized CRISPR-Cas9 technology provide reliable in vitro models for investigating gene function, signaling pathways, disease mechanisms, and therapeutic targets.

PHKA1 (phosphorylase kinase regulatory subunit alpha 1) encodes the skeletal muscle alpha subunit of phosphorylase kinase, a multisubunit enzyme complex that regulates glycogen breakdown. The alpha subunit has a regulatory role within the complex, whose catalytic gamma subunit phosphorylates glycogen phosphorylase to promote glycogenolysis. PHKA1 therefore contributes to regulation of muscle glycogen metabolism, and defects in this gene cause X-linked muscle glycogen storage disease.

Each knockout cell line is validated by STR authentication, sterility testing (bacteria/fungi), and mycoplasma screening. Genotype is confirmed by two rounds of PCR and Sanger sequencing. Only clones that pass all QC criteria are released, ensuring reliable identity and performance for downstream applications.