PIKFYVE Knockout cell line (HAP1)

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PIKFYVE Knockout cell line (HAP1) Order

PIKFYVE Knockout cell line (HAP1)
Catalog#
YKO-H1177
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
PIKFYVE Knockout cell line (HAP1)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
PIKFYVE Knockout cell line (HAP1)
Cell Morphology
 Adherent
Passage ratio
1:5-1:8
Species
Human
Gene Name
PIKFYVE
Gene ID
200576
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%IMDM+10%FBS
Target region
Knockout region: Exon 7 (based on transcript PIKFYVE-201)
Validation: PCR+Sanger Sequencing
PIKFYVE Knockout cell line (HAP1) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PIKFYVE gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HAP1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PIKFYVE gene knockout PIKFYVE Knockout cell line (HAP1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
PIKFYVE Knockout cell line (HAP1)
PIKFYVE Knockout cell line (HAP1)
Catalog#
YKO-H1177
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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Gene Knockout kit

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  • PIKFYVE Knockout cell line (Jurkat)

    PIKFYVE Knockout cell line (Jurkat)

    Organism: Human
    Gene Name: PIKFYVE
    Gene ID: 200576
    Size: 1*10^6
    Catalog#: YKO-HT11167
    Request a Quote More details
  • PIKFYVE Knockout cell line (Hep G2)

    PIKFYVE Knockout cell line (Hep G2)

    Organism: Human
    Gene Name: PIKFYVE
    Gene ID: 200576
    Size: 1*10^6
    Catalog#: YKO-HT3483
    Request a Quote More details
  • PIKFYVE Knockout cell line (THP-1)

    PIKFYVE Knockout cell line (THP-1)

    Organism: Human
    Gene Name: PIKFYVE
    Gene ID: 200576
    Size: 1*10^6
    Catalog#: YKO-HT0700
    Request a Quote More details

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