POLL Knockout cell line (143B)

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POLL Knockout cell line (143B) Order

POLL Knockout cell line (143B)
Catalog#
YKO-HA399
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
POLL Knockout cell line (143B)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
POLL Knockout cell line (143B)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1：2-1：3
Species
Human
Gene Name
POLL
Gene ID
27343
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:143B		 STR Info (Cell bank)
Cell Line:143B
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 13131313
D13S317 12121212
D7S820 11121112
D16S539 10131013
VWA 18181818
TH01 6666
AMEL XXXX
TPOX 11111111
CSF1PO 12121212
D12S391 2020
FGA 2424
D2S1338 2425
D21S11 31.231.2
D18S51 1717
D8S1179 1114
D3S1358 1515
D6S1043 1818
PENTAE 712
D19S433 1313
PENTAD 3910.0
D1S1656 1315
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the POLL gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the 143B cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, POLL gene knockout POLL Knockout cell line (143B) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
POLL Knockout cell line (143B)
POLL Knockout cell line (143B)
Catalog#
YKO-HA399
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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