PRKDC 基因敲除细胞(HEK293)

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PRKDC Knockout cell line(HEK293) Order

PRKDC 基因敲除细胞(HEK293)
Catalog#
YKO-H609
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
PRKDC 基因敲除细胞(HEK293)
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Detailed Product Information

Product category
KO Cell Lines
Cell name
PRKDC Knockout cell line (HEK293)
Cell Morphology
上皮样, 贴壁细胞
Passage ratio
1:3~1:6
Species
Human
Gene Name
PRKDC
Gene ID
5591
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10% FBS
收录情况
该细胞已被Expasy
ExPASy Cellosaurus数据库
该在线资源收录了ATCC、ECACC、DSMZ等多个权威细胞库的细胞系信息，可提供10万+细胞系详尽数据，包括细胞系描述、来源、特性等，具有广泛的参考价值和科学可信度。它还提供细胞系认证、STR数据及潜在污染风险等信息，帮助研究人员确认其实验中所使用的细胞系的可靠性。
收录： CVCL_D9PU
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
Target region
Knockout region: E1 (based on transcript PRKDC-201)
Validation: PCR+Sanger Sequencing
PRKDC 基因敲除细胞(HEK293) Target region
STR Profile
Click to download the STR appraisal report
Loci 送检细胞STR信息
送检细胞名：HEK293		 细胞库细胞STR信息
细胞库细胞名：HEK-293.2sus
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 8888
D13S317 12141214
D7S820 11121112
D16S539 913913
VWA 16191619
TH01 79.379.3
AMEL XXXX
TPOX 11111111
CSF1PO 12121212
D12S391 1921
FGA 2323
D2S1338 1919
D21S11 2830.2
D18S51 1718
D8S1179 1214
D3S1358 1517
D6S1043 1111
PENTAE 715
D19S433 1818
PENTAD 910
D1S1656 1517.3
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

采用独家红棉系统（www.rc-crispr.com）设计PRKDC基因的sgRNA片段敲除方案，将两条sgRNA构建到CRISPR-U™载体上，通过电转法/病毒法将载体转染进HEK293细胞中，使用极限稀释法制备单克隆，并利用EZ-editor™单克隆基因型鉴定试剂盒（货号：YK-MV-1000）对单克隆进行核酸裂解及PCR扩增，再通过Sanger 测序验证基因型，复检合格后对PRKDC基因敲除HEK293细胞进行扩增和冻存。
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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