PSAP Knockout cell line (Jurkat)

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PSAP Knockout cell line (Jurkat) Order

PSAP Knockout cell line (Jurkat)
Catalog#
YKO-HT9070
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
PSAP Knockout cell line (Jurkat)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
PSAP Knockout cell line (Jurkat)
Species
Human
Gene Name
PSAP
Gene ID
5660
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
Target region
Knockout region: Exon 2 (based on transcript PSAP-201)
Validation: PCR+Sanger Sequencing
PSAP Knockout cell line (Jurkat) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PSAP gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Jurkat cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PSAP gene knockout PSAP Knockout cell line (Jurkat) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
PSAP Knockout cell line (Jurkat)
PSAP Knockout cell line (Jurkat)
Catalog#
YKO-HT9070
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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