Ppard Knockout cell line (MC38)

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Ppard Knockout cell line (MC38) Order

Ppard Knockout cell line (MC38)
Catalog#
YKO-M292
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Ppard Knockout cell line (MC38)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
Ppard Knockout cell line (MC38)
Cell Morphology
Epithelial-like, adheren
Passage ratio
1：2-1：4
Species
Mouse
Gene Name
Ppard
Gene ID
19015
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90% DMEM+10%FBS
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
Target region
Knockout region: Exon 3 (based on transcript Ppard-201)
Validation: PCR+Sanger Sequencing
Ppard Knockout cell line (MC38) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:MC38		 STR Info (Cell bank)
Cell Line:MC-38[EXPASY kerafast]
Allele1Allele2Allele3Allele4Allele1Allele2Allele3
18-3 15.016.015.016.0
4-2 20.320.3
6-7 15.016.015.016.0
19-2 13.013.0
1-2 19.018.019.020.0
7-1 26.226.2
8-1 16.016.0
1-1 16.017.015.016.017.0
3-2 13.014.015.014.015.0
2-1 16.016.0
15-3 22.323.322.323.3
6-4 18.018.0
13-1 17.017.0
11-2 16.016.0
17-2 15.016.015.016.0
12-1 17.017.0
5-5 16.017.016.017.0
X-1 27.027.0
TH01
D5S818
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Ppard gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the MC38 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Ppard gene knockout Ppard Knockout cell line (MC38) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
Ppard Knockout cell line (MC38)
Ppard Knockout cell line (MC38)
Catalog#
YKO-M292
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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