Ptgs2 Knockout cell line (RAW 264.7)

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Ptgs2 Knockout cell line (RAW 264.7) Order

Ptgs2 Knockout cell line (RAW 264.7)
Catalog#
YKO-M295
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Ptgs2 Knockout cell line (RAW 264.7)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
Ptgs2 Knockout cell line (RAW 264.7)
Cell Morphology
monocyte/macrophage,adherent
Passage ratio
1:2-1:3
Species
Mouse
Gene Name
Ptgs2
Gene ID
19225
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
Target region
Knockout region: Exon 5 (based on transcript Ptgs2-201)
Validation: PCR+Sanger Sequencing
Ptgs2 Knockout cell line (RAW 264.7) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:RAW 264.7		 STR Info (Cell bank)
Cell Line:RAW 264.7
Allele1Allele2Allele3Allele4Allele1Allele2Allele3
18-3 18.018.0
4-2 22.322.3
6-7 12.012.0
19-2
1-2 17.0
7-1 25.225.2
8-1 13.013.0
1-1 15.016.0
3-2 14.014.0
2-1 16.016.0
15-3 22.322.3
6-4 18.018.0
13-1 16.216.2
11-2 17.017.0
17-2 14.016.014.016.0
12-1 16.016.0
5-5 14.014.0
X-1 24.024.0
TH01
D5S818
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Ptgs2 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the RAW 264.7 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Ptgs2 gene knockout Ptgs2 Knockout cell line (RAW 264.7) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
Ptgs2 Knockout cell line (RAW 264.7)
Ptgs2 Knockout cell line (RAW 264.7)
Catalog#
YKO-M295
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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