RNF149 Knockout cell line (Jurkat)

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RNF149 Knockout cell line (Jurkat) Order

RNF149 Knockout cell line (Jurkat)
Catalog#
YKO-HT9935
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
RNF149 Knockout cell line (Jurkat)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
RNF149 Knockout cell line (Jurkat)
Species
Human
Gene Name
RNF149
Gene ID
284996
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
Target region
Knockout region: Exon 1 (based on transcript RNF149-201)
Validation: PCR+Sanger Sequencing
RNF149 Knockout cell line (Jurkat) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the RNF149 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Jurkat cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, RNF149 gene knockout RNF149 Knockout cell line (Jurkat) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
RNF149 Knockout cell line (Jurkat)
RNF149 Knockout cell line (Jurkat)
Catalog#
YKO-HT9935
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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Gene Knockout kit

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    Organism: Human
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    Request a Quote More details
  • RNF149 Knockout cell line (HCT 116)

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    Size: 1*10^6
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