SIRT7 Knockout cell line (THP-1)

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SIRT7 Knockout cell line (THP-1) Order

SIRT7 Knockout cell line (THP-1)
Catalog#
YKO-HT0399
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
SIRT7 Knockout cell line (THP-1)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
SIRT7 Knockout cell line (THP-1)
Species
Human
Gene Name
SIRT7
Gene ID
51547
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
Target region
Knockout region: Exon 4 (based on transcript SIRT7-201)
Validation: PCR+Sanger Sequencing
SIRT7 Knockout cell line (THP-1) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the SIRT7 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the THP-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, SIRT7 gene knockout SIRT7 Knockout cell line (THP-1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
SIRT7 Knockout cell line (THP-1)
SIRT7 Knockout cell line (THP-1)
Catalog#
YKO-HT0399
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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    Organism: Human
    Gene Name: SIRT7
    Gene ID: 51547
    Size: 1*10^6
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    SIRT7 Knockout cell line (Hep G2)

    Organism: Human
    Gene Name: SIRT7
    Gene ID: 51547
    Size: 1*10^6
    Catalog#: YKO-HT4613
    Request a Quote More details
  • SIRT7 Knockout cell line (HeLa)

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    Gene Name: SIRT7
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    Size: 1*10^6
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