STT3A Knockout cell line (Jurkat)

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STT3A Knockout cell line (Jurkat) Order

STT3A Knockout cell line (Jurkat)
Catalog#
YKO-HT10531
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
STT3A Knockout cell line (Jurkat)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
STT3A Knockout cell line (Jurkat)
Species
Human
Gene Name
STT3A
Gene ID
3703
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
Target region
Knockout region: Exon 5 (based on transcript STT3A-201)
Validation: PCR+Sanger Sequencing
STT3A Knockout cell line (Jurkat) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the STT3A gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Jurkat cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, STT3A gene knockout STT3A Knockout cell line (Jurkat) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
STT3A Knockout cell line (Jurkat)
STT3A Knockout cell line (Jurkat)
Catalog#
YKO-HT10531
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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