TNRC6A Knockout cell line (Hep G2)

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TNRC6A Knockout cell line (Hep G2) Order

TNRC6A Knockout cell line (Hep G2)
Catalog#
YKO-HT4238
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
TNRC6A Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
TNRC6A Knockout cell line (Hep G2)
Species
Human
Gene Name
TNRC6A
Gene ID
27327
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 5 (based on transcript TNRC6A-202)
Validation: PCR+Sanger Sequencing
TNRC6A Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the TNRC6A gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, TNRC6A gene knockout TNRC6A Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
TNRC6A Knockout cell line (Hep G2)
TNRC6A Knockout cell line (Hep G2)
Catalog#
YKO-HT4238
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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    TNRC6A Knockout cell line (U-2 OS)

    Organism: Human
    Gene Name: TNRC6A
    Gene ID: 27327
    Size: 1*10^6
    Catalog#: YKO-HT13321
    Request a Quote More details
  • TNRC6A Knockout cell line (Jurkat)

    TNRC6A Knockout cell line (Jurkat)

    Organism: Human
    Gene Name: TNRC6A
    Gene ID: 27327
    Size: 1*10^6
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    Request a Quote More details
  • TNRC6A Knockout cell line (HeLa)

    TNRC6A Knockout cell line (HeLa)

    Organism: Human
    Gene Name: TNRC6A
    Gene ID: 27327
    Size: 1*10^6
    Catalog#: YKO-XN54628
    Request a Quote More details

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