Vgf Knockout cell line (4T1)

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Vgf Knockout cell line (4T1) Order

Vgf Knockout cell line (4T1)
Catalog#
YKO-M298
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Vgf Knockout cell line (4T1)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
Vgf Knockout cell line (4T1)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Mouse
Gene Name
Vgf
Gene ID
381677
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
88%RPMI-1640+10% FBS+1%Glutamax+1%Sodium Pyruvate
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
Target region
Knockout region: Exon 5 (based on transcript Vgf-204)
Validation: PCR+Sanger Sequencing
Vgf Knockout cell line (4T1) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:4T1		 STR Info (Cell bank)
Cell Line:4T1
Allele1Allele2Allele3Allele4Allele1Allele2Allele3
18-3 18.019.018.019.0
4-2 21.321.3
6-7 12.012.0
19-2 13.013.0
1-2 17.017.0
7-1 25.225.2
8-1 13.013.0
1-1 15.016.015.016.0
3-2 14.015.014.015.0
2-1 16.017.016.017.0
15-3 22.322.3
6-4 18.018.0
13-1 16.216.2
11-2 18.020.018.019.020.0
17-2 15.015.0
12-1 16.016.0
5-5 14.014.0
X-1 25.025.0
TH01
D5S818
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Vgf gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the 4T1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Vgf gene knockout Vgf Knockout cell line (4T1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
Vgf Knockout cell line (4T1)
Vgf Knockout cell line (4T1)
Catalog#
YKO-M298
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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