ZW10 Knockout cell line (A549)

CRISPR knockout cell lines are engineered through targeted genome editing to eliminate gene function, supporting studies of cellular biology, disease mechanisms, and drug discovery.

ZW10 (zw10 kinetochore protein) encodes a kinetochore-associated protein that contributes to accurate chromosome segregation during cell division. ZW10 is an essential component of the mitotic checkpoint, which prevents premature progression through mitosis when chromosome attachment is incomplete or incorrect. Its function therefore supports chromosome stability and faithful distribution of genetic material to daughter cells.

A549 is a human lung cancer cell line established in 1972 from an explanted lung tumor of a 58-year-old male patient. The cells exhibit characteristics of alveolar type II pulmonary epithelial cells, including the ability to synthesize lecithin through the cytidine diphosphocholine pathway. A549 cells have been described as originating from lung adenocarcinoma and are widely used as a model for studying lung cancer biology, pulmonary epithelial function, drug responses, and respiratory-related research.

Comprehensive quality control includes STR authentication, sterility testing (bacteria and fungi), and mycoplasma screening to ensure purity and performance. Genotype is confirmed by two rounds of PCR and Sanger sequencing, and complete validation reports are provided for make-to-order products.