ZW10 Knockout cell line (AC16)

CRISPR knockout cell lines are engineered through targeted genome editing to eliminate gene function, supporting studies of cellular biology, disease mechanisms, and drug discovery.

ZW10 (zw10 kinetochore protein) encodes a kinetochore-associated protein that contributes to accurate chromosome segregation during cell division. ZW10 is an essential component of the mitotic checkpoint, which prevents premature progression through mitosis when chromosome attachment is incomplete or incorrect. Its function therefore supports chromosome stability and faithful distribution of genetic material to daughter cells.

Human cardiac ventricular cells are represented by AC16, an immortalized cell line derived from primary human ventricular tissue. The cells were generated by fusion of primary adult ventricular cardiomyocytes with a human fibroblast-like cell line carrying a temperature-sensitive SV40 large T antigen. AC16 exhibits cardiac-related characteristics and is used as an in vitro model for studies of human cardiomyocyte biology.

Comprehensive quality control includes STR authentication, sterility testing (bacteria and fungi), and mycoplasma screening to ensure purity and performance. Genotype is confirmed by two rounds of PCR and Sanger sequencing, and complete validation reports are provided for make-to-order products.