ZW10 Knockout cell line (Caco-2)

CRISPR knockout cell lines are engineered through targeted genome editing to eliminate gene function, supporting studies of cellular biology, disease mechanisms, and drug discovery.

ZW10 (zw10 kinetochore protein) encodes a kinetochore-associated protein that contributes to accurate chromosome segregation during cell division. ZW10 is an essential component of the mitotic checkpoint, which prevents premature progression through mitosis when chromosome attachment is incomplete or incorrect. Its function therefore supports chromosome stability and faithful distribution of genetic material to daughter cells.

Caco-2 is a human colorectal adenocarcinoma cell line established from a primary colon tumor of a 72-year-old male patient. The cells can form differentiated intestinal epithelial-like monolayers with characteristics including microvilli, tight junctions, dome formation, and transport functionality. Caco-2 cells also produce mucus and can form moderately differentiated adenocarcinoma-like tumors in nude mice. The cell line is widely used as an in vitro model for studying intestinal epithelial differentiation, barrier function, permeability, drug absorption, and colorectal cancer biology.

Each knockout cell line is validated by STR authentication, sterility testing (bacteria/fungi), and mycoplasma screening. Genotype is confirmed by two rounds of PCR and Sanger sequencing. Only clones that pass all QC criteria are released, ensuring reliable identity and performance for downstream applications.