NCI-H1299-Luc cell line

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NCI-H1299-Luc Order

NCI-H1299-Luc cell line
Catalog#
YC-D010-Luc-P
Size
1*10^6
Transfection Culture Medium
Transfection Culture Medium(NCI-H1299)
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Datasheet
NCI-H1299-Luc cell line
Order

Detailed Product Information

Product category
Luciferase Stable Cell Lines
cell name
NCI-H1299-Luc
Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:3
Species
Human
Reporter gene
Luciferase
Resistance gene
puro
Construction method
Lentivirus
Mycoplasma test
negative
Culture method
88%RPMI-1640+10% FBS+1%Glutamax+1%Sodium Pyruvate
Accession
CVCL_C8W0(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Luciferase validation
Sample Compound
pore 1		 Compound
pore 2		 Compound
pore 3		 Average Expressed
multiple
NCI-H1299-Luc 187146 170651 185028 180941.667 333.636
NCI-H1299 489 589 549 542.333
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:NCI-H1299		 STR Info (Cell bank)
Cell Line:NCI-H1299-Luc2-tdT-2
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11111111
D13S317 12121212
D7S820 10101010
D16S539 12131213
VWA 16181618
TH01 69.369.3
AMEL XXXX
TPOX 8888
CSF1PO 12121212
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use luciferase gene expressing lentivirus constructed by Ubigene to infect the NCI-H1299 cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a NCI-H1299-Luc cell line that can maintain long-term, stable and high expression.

Product Advantage

  • Easy to use: Luciferase Stable Cell Line in our cell bank can stably express luciferase. It can be directly used for in vivo injection to detect the tumorigenicity of cells.
    01
  • Sensitive accurate：Luciferase Stable Cell Line in our cell bank have high specificity and high sensitivity, enables high imaging quality, and its luminescence intensity can be accurately quantified.
    02
  • High-quality cell: Our Luciferase Stable Cell Lines have been optimized and have low passages, high activity and good cell condition, applicable for detection or tumorigenesis experiment.
    03

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