Name: 3D4/21-Luc
SKU: 1*10^6
Price: 1590 USD
Availability: InStock

Red cotton OmniCell bank

10000⁺

Gene Knockout Cell Lines

2000⁺

Luc/EGFP/Cas9 Tool Cell Lines

1000⁺

Wild-type Cell Lines

3D4/21-Luc

Copy Product Info

Catalog#

YC-C041-Luc-P

Size

1*10^6 Cells/vial (frozen vial)

Culture method

85%RPMI-1640+10%FBS+1%Glutamax+1%SodiumPyruvate+1%NEAA+1%Hepes+2.2g/L Glucose

Price(USD)

$1590

Instruction

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Overview

Gene reporter systems are widely used in studies of eukaryotic gene expression and cellular physiology, serving as a common approach to improving experimental accuracy and reliability. The luciferase reporter system, which detects luciferase activity using luciferin or firefly aldehyde as substrates, is one of the most extensively applied reporter systems. Owing to its operational simplicity, high sensitivity, and high success rate, it is widely applied in gene expression research.

The 3D4/21-Luciferase cell line developed by Ubigene was generated using the lentivirus method. In addition to enabling stable and high-efficiency expression of the luciferase gene, it offers high specificity, excellent imaging quality, and accurately quantifiable luminescence intensity. This cell line supports a broad range of downstream applications, including promoter activity analysis, mammalian two-hybrid assays, and in vivo animal imaging studies. Its flexibility and quantitative performance make it reliable for gene regulation research, functional validation, and preclinical model development.

Detailed Product Information

3D4/21-Luc

Product name

3D4/21-Luc

Reporter gene

Firefly Luciferase

Catalog number

YC-C041-Luc-P

Resistance gene

Puromycin

Product category

Luciferase Stable Cell Lines

Mycoplasma test

Negative

Species

Pig

Accession

Cell morphology

Macrophage-like, adherent

Shipping condition

Frozen vials; dry-ice package

Passage ratio

1:2~1:3

Storage condition

Liquid nitrogen

Culture method

85%RPMI-1640+10%FBS+1%Glutamax+1%SodiumPyruvate+1%NEAA+1%Hepes+2.2g/L Glucose

Intended use

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

Background

The parental porcine monomyeloid cell line, 3D4, was established in December of 1998 following transfection of primary porcine alveolar macrophage cultures with the pSV3neo plasmid. Single cell cloning and selection in G-418 of the 3D4 parental cell line resulted in establishment of 3D4/2 (ATCC CRL-2845), 3D4/21 (ATCC CRL-2843) and 3D4/31 (ATCC CRL-2844).

Product Advantages

Easy to use

Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations

High-efficient KO

These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.

Curated cells

Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.

Plasmid Backbone Map

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Quality Control

1. Bacteria and fungi test

None Detected.

2. Mycoplasma test

Negative.

4. Luciferase stable expression validation

In vivo imaging process

Figure 1. In vivo imaging process of luciferase stable tumor cell line

Luciferase validation process

Luciferase activity was detected using the Dual-Luciferase® Reporter Assay System Kit(Promega, Cat: E1910), and the firefly luciferase reaction intensity was read in the Microplate Reader (BioTek, Synergy LX).