Name: Porcine Alveolar Macrophage Cell Line (3D4/21)
SKU: 1*10^6
Price: 535 USD
Availability: InStock

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10000⁺

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2000⁺

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Wild-type Cell Lines

Porcine Alveolar Macrophage Cell Line (3D4/21)

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Catalog#

YC-C041

Size

1*10^6 Cells/vial (frozen vial)

Culture method

85%RPMI-1640+10%FBS+1%Glutamax+1%SodiumPyruvate+1%NEAA+1%Hepes+2.2g/L Glucose

Instruction

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Overview

The parental porcine monomyeloid cell line, 3D4, was established in December of 1998 following transfection of primary porcine alveolar macrophage cultures with the pSV3neo plasmid. Single cell cloning and selection in G-418 of the 3D4 parental cell line resulted in establishment of 3D4/2 (ATCC CRL-2845), 3D4/21 (ATCC CRL-2843) and 3D4/31 (ATCC CRL-2844).

Detailed Product Information

Porcine Alveolar Macrophage Cell Line (3D4/21)

This cell line has a well-defined origin, a clean background, and broad applicability. It can be reliably used in basic research applications such as functional validation, signaling pathway analysis, and drug treatment.

Product name

Porcine Alveolar Macrophage Cell Line (3D4/21)

Mycoplasma test

Negative

Catalog number

YC-C041

Cellosaurus ID

CVCL_0F14

Product category

Wild-type Cell Lines

Shipping condition

Frozen vials; dry-ice package

Cell morphology

Macrophage-like, adherent

Storage condition

Liquid nitrogen

Culture Medium

85%RPMI-1640+10%FBS+1%Glutamax+1%SodiumPyruvate+1%NEAA+1%Hepes+2.2g/L Glucose

Species/Source

Porcine Alveolar Macrophage Cell Line

Passage ratio

1:2~1:3

Intended use

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

Sterility Testing

All cells have been tested and are free of bacteria, viruses, mycoplasma, and other toxins.

Recommended transfection method

Electroporation

Lentivirus

Quality Control Specifications

1. Bacteria and fungi test

The test result was negative.

2. Mycoplasma test

The test result was negative.

Intended use

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use

Unpacking and Storage Instructions

1.

Cell Reception

2.

Unpacking and storage instructions

Handling procedure

1.

Cell Thawing

2.

Cell Passaging

3.

Cell cryopreservation

References

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FAQ

Q1. How to Contact Support if Issues Arise After Receiving the KO Cells?

If any abnormalities are observed after receiving the cells, please provide the following information based on the stage of use and contact us promptly for assistance:

(1) Issues upon delivery: Please provide photos of the cells at the time of receipt along with a brief description of the issue. We will offer after-sales support accordingly.

(2) Issues after thawing or passaging: Please provide microscopic images of the cells before and after thawing/passaging, along with a detailed description of the experimental procedures. Our technical team will provide professional support.

Q2. What Information Is Required When Reporting KO Cell Product Issues?

Q3. How Should the KO cell Product Be Stored Upon Receipt?

Q4. Shipping Method

Q5. Are the Knockout Cell Lines Stable for Passaging? Is Authentication Provided?