ARPE-19 cell line

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location： Home > Products > Wild-type Cell Lines > Human Retinal Pigment Epithelial Cell Line(ARPE-19)
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  • Wild-type Cell Lines-1 Human Retinal Pigment Epithelial Cell Line(ARPE-19)-Wild-type Cell Lines-1
  • Wild-type Cell Lines-2 Human Retinal Pigment Epithelial Cell Line(ARPE-19)-Wild-type Cell Lines-2
  • Transfection Human Retinal Pigment Epithelial Cell Line(ARPE-19)-Transfection
  • Monoclonal Human Retinal Pigment Epithelial Cell Line(ARPE-19)-Monoclonal
  • ARPE-19 cell line Human Retinal Pigment Epithelial Cell Line(ARPE-19)-产品图
Wild-type Cell Lines-1 Human Retinal Pigment Epithelial Cell Line(ARPE-19)-Wild-type Cell Lines-1
Wild-type Cell Lines-2 Human Retinal Pigment Epithelial Cell Line(ARPE-19)-Wild-type Cell Lines-2
Transfection Human Retinal Pigment Epithelial Cell Line(ARPE-19)-Transfection
Monoclonal Human Retinal Pigment Epithelial Cell Line(ARPE-19)-Monoclonal
ARPE-19 cell line Human Retinal Pigment Epithelial Cell Line(ARPE-19)-产品图
Human Retinal Pigment Epithelial Cell Line(ARPE-19) Copy Product Info
Catalog#： YC-C033
Size： 1*10^6
Culture method： 90% DMEM/F12+10% FBS
STR Report
Datasheet (Use Instruction)
  • Wild-type Cell Lines-1 Human Retinal Pigment Epithelial Cell Line(ARPE-19)-Wild-type Cell Lines-1
  • Wild-type Cell Lines-2 Human Retinal Pigment Epithelial Cell Line(ARPE-19)-Wild-type Cell Lines-2
  • Transfection Human Retinal Pigment Epithelial Cell Line(ARPE-19)-Transfection
  • Monoclonal Human Retinal Pigment Epithelial Cell Line(ARPE-19)-Monoclonal
  • ARPE-19 cell line Human Retinal Pigment Epithelial Cell Line(ARPE-19)-产品图
Human Retinal Pigment Epithelial Cell Line(ARPE-19) Copy Product Info
Catalog#： YC-C033
Size： 1*10^6
Culture method： 90% DMEM/F12+10% FBS
Datasheet (Use Instruction)

Detailed Product Information

Cell Info

Product category Wild-type Cell Lines Mycoplasma test negative
Cell Morphology Endothelial cells, adherent Culture method 90% DMEM/F12+10% FBS
Passage ratio 1：2 Species Human
Accession
CVCL_0145(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Product category
Wild-type Cell Lines
Cell Morphology
Endothelial cells, adherent
Passage ratio
1：2
Mycoplasma test
negative
Culture method
90% DMEM/F12+10% FBS
Species
Human
Accession
CVCL_0145(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
ARPE-19 is a spontaneously arising retinal pigment epithelia (RPE) cell line derived in 1986 by Amy Aotaki-Keen from the normal eyes of a 19-year-old male who died from head trauma in a motor vehicle accident.The line was established in a 1:1 mixture of Dulbecco's modified Eagles medium and Ham's F12 medium with HEPES buffer containing 20% fetal bovine serum, 56 mM final concentration sodium bicarbonate and 2 mM L-glutamine and incubated at 37C in 10% CO2. The cells were subjected to selective trypsinization for the first four passages to remove superficial cells before passaging the cuboidal basal layer. By passage 5, the cultures appeared to be rapidly growing RPE cells, which would form cobblestone monolayers, which pigmented after several months in culture.

Recommended Transfection Method

Electroporation
Lentivirus Transduction
STR Profile
Download STR Report
Loci STR Info (Sample Cell)
Sample Cell Line:ARPE-19		 STR Info (Cell bank)
Cell Line:ARPE-19
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 13131313
D13S317 11121112
D7S820 911911
D16S539 911911
VWA 16191619
TH01 69.369.3
AMEL XYXY
TPOX 911911
CSF1PO 11111111
D12S391 2122
FGA 2323
D2S1338 1919
D21S11 2829
D18S51 1216
D8S1179 1313
D3S1358 1415
D6S1043 1719
PENTAE 711
D19S433 1213
PENTAD 1113
D1S1656 1115
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.
Related Resources: Red Cotton Gene-editing System | ATCC | DSMZ | ECAZZ | Cellosaurus | CCTCC | NCBI
Learn More>>>

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