CALCA Stable Expression Cell Line (HCE-T)

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CALCA Stable Expression Cell Line (HCE-T)

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CALCA Stable Expression Cell Line (HCE-T)

CALCA Stable Expression Cell Line (HCE-T) Copy Product Info

Catalog#： YOE-L090

Size： 1*10^6

Culture method： 85%DMEM/F12+15%FBS+5ug/ml insulin+10ng/ml human EGF

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STR Report

Datasheet (Use Instruction)

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CALCA Stable Expression Cell Line (HCE-T)

CALCA Stable Expression Cell Line (HCE-T) Copy Product Info

Catalog#： YOE-L090

Size： 1*10^6

Culture method： 85%DMEM/F12+15%FBS+5ug/ml insulin+10ng/ml human EGF

Order

STR Report

Datasheet (Use Instruction)

Detailed Product Information

Cell Info

Catalog	YOE-L090	Transcript	NM_001741.3
Cell name	CALCA Stable Expression Cell Line (HCE-T)	Species	Human
Gene Name	CALCA	Passage ratio	1：2-1：5
Cell Morphology	Epithelial-like, adherent	Antibiotic Concentration	Puro=1μg/ml
Culture method	85%DMEM/F12+15%FBS+5ug/ml insulin+10ng/ml human EGF	Mycoplasma test	negative

Catalog

YOE-L090

Cell name

CALCA Stable Expression Cell Line (HCE-T)

Gene Name

CALCA

Cell Morphology

Epithelial-like, adherent

Passage ratio

1：2-1：5

Transcript

NM_001741.3

Species

Human

Antibiotic Concentration

Puro=1μg/ml

Mycoplasma test

negative

Culture method

85%DMEM/F12+15%FBS+5ug/ml insulin+10ng/ml human EGF

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

Product Description

This gene encodes the peptide hormones calcitonin, calcitonin gene-related peptide and katacalcin by tissue-specific alternative RNA splicing of the gene transcripts and cleavage of inactive precursor proteins. Calcitonin is involved in calcium regulation and acts to regulate phosphorus metabolism. Calcitonin gene-related peptide functions as a vasodilator and as an antimicrobial peptide while katacalcin is a calcium-lowering peptide. Multiple transcript variants encoding different isoforms have been found for this gene.

Gene Expression Validation

No.	Sample	hCALCA-1CтMean	β-h_actinCтMean	ΔCтMean	ΔΔCт	Expression Rate
1	CALCA Stable Expression Cell Line (HCE-T)	13.835	14.87	-1.035	-13.113	8859.441
2	HCE-T_Ctrl(Puro)	26.895	14.817	12.078	0.000	1.000

STR Profile

Download STR Report

Loci	STR Info (Sample Cell) Sample Cell Line:HCE-T			STR Info (Cell bank) Cell Line:HCE-T
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	13	13		13	13
D13S317	8	8		8	8
D7S820	10	13		10	13
D16S539	9	11		9	11
VWA	18	19		18	19
TH01	7	8		7	8
AMEL	X	X		X	X
TPOX	8	11		8	11
CSF1PO	12	12		12	12
D12S391	18	19
FGA	20	20
D2S1338	19	20
D21S11	27	30
D18S51	13	15
D8S1179	11	13
D3S1358	17	17
D6S1043	18	19
PENTAE	15	16
D19S433	13	14
PENTAD	12	15
D1S1656	13	15

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

High expression

Exclusive cell selection technique, ensuing high expression, RT-qPCR validation data provided.
Good stability

Stable gene copy number, low passage, can be stably passaged for more than 20 passages.
Strict QC

Strict quality control, ensuing no bacterial and mycoplasma contamination, STR authentication and cell post-thawing viability test passed; High standard delivery.

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