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PADI2 Stable Expression Cell Line (HCT 116)

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PADI2 Stable Expression Cell Line (HCT 116)
Size
1*10^6
Culture method
90%McCOY's 5A+10% FBS
Instruction
STR Report
Detailed Product Information
PADI2 Stable Expression Cell Line (HCT 116)

This gene encodes a member of the peptidyl arginine deiminase family of enzymes, which catalyze the post-translational deimination of proteins by converting arginine residues into citrullines in the presence of calcium ions. The family members have distinct substrate specificities and tissue-specific expression patterns. The type II enzyme is the most widely expressed family member. Known substrates for this enzyme include myelin basic protein in the central nervous system and vimentin in skeletal muscle and macrophages. This enzyme is thought to play a role in the onset and progression of neurodegenerative human disorders, including Alzheimer disease and multiple sclerosis, and it has also been implicated in glaucoma pathogenesis. This gene exists in a cluster with four other paralogous genes.

Product category
Stable Cell Lines
Transcript
NM_007365.3
Cell Name
PADI2 Stable Expression Cell Line (HCT 116)
Species
Human
Gene Name
PADI2
Passage ratio
1:2~1:4
Cell Morphology
Epithelial-like, adherent
Antibiotic Concentration
Puro=0.5μg/ml
Culture method
90%McCOY's 5A+10% FBS
Mycoplasma test
negative
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
Gene Expression Validation
Sample NamehPADI2-1CтMeanβ-h_actin CтMeanΔCтMeanΔΔCтExpression Rate
PADI2 Stable Expression Cell Line (HCT 116)19.47414.1475.327-8.521367.347
HCT116_Ctrl(Puro)29.72115.87313.8480.0001.000

Intended use

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use

Unpacking and Storage Instructions

1.
Cell Reception
2.
Unpacking and storage instructions

Handling procedure

1.
Cell Thawing
2.
Cell Passaging
3.
Cell cryopreservation

Product Advantage

Easy to use
Easy to use

Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations

High-efficient KO
High-efficient KO

These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.

Curated cells
Curated cells

Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.

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