Expert Insights - CRISPR Library

Leveraging Ubigene CRISPR Library Cell Pools for High-Throughput Functional Genomics

In recent years, CRISPR/Cas9 technology has revolutionized functional genomics, providing researchers with a robust platform to interrogate gene function systematically across the genome. As the demand for high-throughput, reliable, and reproducible screening systems increases, Ubigene has developed CRISPR Library Cell Pools, powered by proprietary CRISPR iScreen™ technology, to meet the needs of modern genomic research. These screening-ready cell pools combine the power of Cas9-mediated genome editing with comprehensive guide RNA (gRNA) libraries, enabling researchers to explore gene function under a wide range of biological contexts.

Ubigene CRISPR Library Cell Pools are collections of mammalian cells that are stably engineered to express the Cas9 nuclease along with a curated library of gRNAs. Each pool is designed for either genome-wide knockout studies or focused gene-targeted investigations, providing a versatile platform for both broad and hypothesis-driven functional screens. These cell pools are particularly valuable for high-throughput genetic screens, enabling the identification of genes involved in disease mechanisms, drug responses, or cellular stress adaptation.

1. High-Quality Content

Each cell pool is generated from a high-coverage gRNA library, ensuring comprehensive representation of target genes. Ubigene employs rigorous next-generation sequencing (NGS) and quality control procedures to verify library composition, uniformity, and coverage. This attention to detail ensures that every batch of cell pools maintains a high level of consistency, minimizing experimental variability and enhancing reproducibility.

2. Optimized Infection and Genetic Engineering

To achieve efficient and precise genome editing, Ubigene leverages a low multiplicity of infection (MOI) strategy during lentiviral transduction, ensuring that each cell receives a single gRNA construct. This "one virus, one gRNA per cell" approach is critical for interpreting screening data accurately, as it reduces confounding effects from multiple simultaneous edits in a single cell.

3. Exceptional Reproducibility

Standardized manufacturing and rigorous batch-to-batch validation allow researchers to replicate experiments with confidence. By minimizing technical variability, Ubigene CRISPR Library Cell Pools provide a robust foundation for comparative studies, whether across different experimental conditions, cell types, or laboratories.

Ubigene CRISPR Library Cell Pools are versatile tools for a wide range of applications in basic and applied research:

1. Genome-Wide Loss-of-Function Screens: Identify essential genes required for cell viability, proliferation, or differentiation.

2. Targeted Gene Discovery: Focus on specific pathways or gene families to understand their role under physiological or stress conditions.

3. Drug Response Studies: Screen for genes that modulate sensitivity or resistance to small molecules, biologics, or environmental stressors.

4. Mechanistic Studies: Dissect signaling pathways, metabolic networks, or gene regulatory circuits in complex cellular contexts.

By combining Cas9-mediated gene editing with a high-coverage gRNA library, researchers can systematically perturb gene function at scale, providing deep insights into cellular processes and disease mechanisms.

Ubigene's CRISPR Library Cell Pools offer a turnkey solution for functional genomics studies, reducing the time, labor, and technical complexity associated with generating and validating custom knockout libraries. They provide:

• Immediate readiness for high-throughput screens

• Reliable and reproducible gene coverage

• Flexibility for both genome-wide and targeted investigations

• Compatibility with a range of mammalian cell types and experimental designs

These characteristics make Ubigene CRISPR Library Cell Pools an ideal platform for both exploratory research and translational studies, enabling the discovery of genes critical to disease, development, and cellular response mechanisms.

In addition to screening-ready cell pools, Ubigene offers custom CRISPR library screening solutions, including genome-wide or pathway-specific gRNA design, lentiviral packaging, and functional validation. Leveraging Ubigene's proprietary CRISPR iScreen™ technology, these services provide enhanced editing efficiency, comprehensive library coverage (≥99%), and rapid deployment, supporting a broad spectrum of research applications from functional genomics to drug target discovery. By combining high-quality CRISPR libraries, stable Cas9-expressing cell lines, and expert technical support, Ubigene enables researchers to accelerate their discovery pipelines and uncover novel insights into gene function across diverse biological systems.

In summary, Ubigene CRISPR Library Cell Pools represent a powerful, reproducible, and versatile platform for functional genomic screening. Whether your research focuses on basic biology, disease mechanisms, or therapeutic target identification, these cell pools and Ubigene's CRISPR library screening services provide the tools and expertise necessary to drive gene function discovery to the next level.

Ubigene offers a comprehensive suite of CRISPR iScreen™ library products to support high-throughput functional genomics studies, from plasmids to viral vectors and screening-ready cell pools. Each product is designed for high coverage (>99%), uniformity (<10), and reproducibility, enabling researchers to perform genome-wide or pathway-specific screens with confidence.

35+ CRISPR iScreen™ Library Plasmid: Plasmid coverage>99%, uniformity<10, Learn More >>

150+ CRISPR iScreen™ Library Virus: Strong activity, high titer, ready-to-use, Learn More >>

400+ CRISPR iScreen™ Library Cell Pool: Cell coverage rate up to 99%, Learn More >>

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