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MFE-280-Luc

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MFE-280-Luc
Copy Product Info
Catalog#
YC-C101-Luc-P
Size
1*10^6 Cells/vial (frozen vial)
Culture method
88%MEM+10%FBS+1%NEAA+1%1%Sodium Pyruvate
Price(USD)
$1290
Instruction
STR Report

Overview

Gene reporter systems are widely used in studies of eukaryotic gene expression and cellular physiology, serving as a common approach to improving experimental accuracy and reliability. The luciferase reporter system, which detects luciferase activity using luciferin or firefly aldehyde as substrates, is one of the most extensively applied reporter systems. Owing to its operational simplicity, high sensitivity, and high success rate, it is widely applied in gene expression research.

The MFE-280-Luciferase cell line developed by Ubigene was generated using the lentivirus method. In addition to enabling stable and high-efficiency expression of the luciferase gene, it offers high specificity, excellent imaging quality, and accurately quantifiable luminescence intensity. This cell line supports a broad range of downstream applications, including promoter activity analysis, mammalian two-hybrid assays, and in vivo animal imaging studies. Its flexibility and quantitative performance make it reliable for gene regulation research, functional validation, and preclinical model development.

Detailed Product Information

MFE-280-Luc
Product name
MFE-280-Luc
Reporter gene
Firefly Luciferase
Catalog number
YC-C101-Luc-P
Resistance gene
Puromycin
Product category
Luciferase Stable Cell Lines
Mycoplasma test
Negative
Species
Human
Accession
-
Cell morphology
Epithelial-like, adherent
Shipping condition
Frozen vials; dry-ice package
Passage ratio
1:2-1:3
Storage condition
Liquid nitrogen
Culture method
88%MEM+10%FBS+1%NEAA+1%1%Sodium Pyruvate
Intended use
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

Product Advantages

Easy to use
Easy to use

Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations

High-efficient KO
High-efficient KO

These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.

Curated cells
Curated cells

Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.

Plasmid Backbone Map

Plasmid Backbone Map

View Picture

Quality Control

1. Bacteria and fungi test
None Detected.
2. Mycoplasma test
Negative.
3. STR Authentication
4. Luciferase stable expression validation
In vivo imaging process
In vivo imaging process
Figure 1. In vivo imaging process of luciferase stable tumor cell line
Luciferase validation process
Luciferase activity was detected using the Dual-Luciferase® Reporter Assay System Kit(Promega, Cat: E1910), and the firefly luciferase reaction intensity was read in the Microplate Reader (BioTek, Synergy LX).
In vivo imaging process
Figure 2. Luciferase activity validation workflow
RT-qPCR Test Results
Sample NameDuplicated well 1Duplicated well 2Duplicated well 3Average valueRatio
MFE-280-Luc271184260484269618267095.333430.336
MFE-280606647609620.667

Intended use

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use

Unpacking and Storage Instructions

1.
Cell Reception
2.
Unpacking and storage instructions

Handling procedure

1.
Cell Thawing
2.
Cell Passaging
3.
Cell cryopreservation

References

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