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Nlrp3 Stable Expression Cell Line (MC3T3-E1)

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Nlrp3 Stable Expression Cell Line (MC3T3-E1)
Size
1*10^6
Culture method
90% Alpha MEM+10%FBS
Instruction
Detailed Product Information
Nlrp3 Stable Expression Cell Line (MC3T3-E1)
Product category
Stable Cell Lines
Transcript
NM_001359638.1
Cell Name
Nlrp3 Stable Expression Cell Line (MC3T3-E1)
Species
Mouse
Gene Name
Nlrp3
Passage ratio
1:3~1:5
Cell Morphology
Fibroblast-like, adherent
Antibiotic Concentration
Puro=1μg/ml
Culture method
90% Alpha MEM+10%FBS
Mycoplasma test
negative
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
Gene Expression Validation
Sample NamemNlrp3-2CтMeanQ-mGAPDHCтMeanΔCтMeanΔΔCтExpression Rate
Nlrp3 Stable Expression Cell Line (MC3T3-E1)21.62214.1187.504-9.401676.056
MC3T3-E1_Ctrl(Puro)31.8414.93516.9050.0001.000

Intended use

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use

Unpacking and Storage Instructions

1.
Cell Reception
2.
Unpacking and storage instructions

Handling procedure

1.
Cell Thawing
2.
Cell Passaging
3.
Cell cryopreservation

Product Advantage

Easy to use
Easy to use

Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations

High-efficient KO
High-efficient KO

These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.

Curated cells
Curated cells

Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.

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