CRISPR library complete solution

CRISPR library is a high-throughput gene screening method established based on CRISPR/Cas9 technology. It identifies phenotype-related genes or screens new drug targets through functional screening, enrichment and deep sequencing analysis. The scope of screening can be the whole genome, a certain gene family, or a certain signal pathway gene. CRISPR library screening has become the preferred platform for large-scale gene function screening benefited from the characteristics of CRISPR/Cas9 such as versatility, low noise, high knockout efficiency, and less off-target effect.

Advantages of Ubigene

Red Cotton CRISPR gene editing system The exclusive data processing system can automatically design 3-6 gRNAs for each gene.

Exclusive CRISPR-U™ technology Gene-editing efficiency elevate 10-20 times due to higher cleavage efficiency and homologous-recombination efficiency.

More than 5000 successful gene-editing cases gene editing has been successfully implemented on more than 200 kinds of cell line. And more than 3000 KO cell lines and 10000 gRNA plasmids have been launched.

Nearly a hundred Cas9 stable cell lines Low cell passage, high cell viability and good cell condition of these cell lines with STR authentication and Cas9 expression detection reports easily accelerate the construction of CRISPR library.

Screening Library Customization

Focusing on gene editing and having rich experience in cell line gene editing, Ubigene can provide 8 major libraries and one-stop services including sgRNA library construction, virus packaging, cell transfection, antibiotic screening, NGS sequencing, and data analysis.

Service Types

Ubigene provides one-stop services for CRISPR-KO, CRISPRa and CRISPRi customized libraries, including sgRNA library construction, virus packaging, cell transfection, antibiotic screening, high-throughput sequencing and data analysis. We offer multiple deliverables meet different research needs.

CRISPR-KO library

Cas9:gRNA complex

The gRNAs of this kind of library mainly target the 5' end exons of coding genes. DNA double strand breaks are caused by Cas9 protein cleavage, and knockout is achieved by introducing frameshift mutations through the non homologous end joining (NHEJ) DNA repair mechanism.

CRISPRa library

dCas9-SAM system

This kind of library can target the transcription-regulatory regions of coding or non-coding genes, achieve the co-expression of Cas9 protein and MS2-P65-HSF1 activation auxiliary protein by connecting dCas9 protein and transcription activator VP64, effectively upregulate the expression level of the genes, and complete high-throughput gain-of-function screening.

CRISPRi library

dCas9-KRAB

The gRNAs of this kind of library mainly target non-coding genes and essential genes (knockout lethality). Gene knockdown was achieved by co-transfecting the gRNA targeting the upstream regulatory region of the gene and a protein of the catalytically inactive dCas9 fused with the transcription repressor KRAB (Kruppel-related frame domain).

Services Details

Technical Process

Libraries in Stock

Ubigene's off-shelf whole genome CRISPR library of human and mouse can achieve high throughput and rapid screening of functional genes of the entire genome. The human whole genome CRISPR libraries include the single plasmid system (gRNA and Cas9 are in the same plasmid) and the dual plasmid system (gRNA and Cas9 are in different plasmids). At present, only single plasmid system of the mouse whole genome CRISPR library is provided. These three libraries are divided into two sub-libraries, A and B. Each library has designed three gRNAs for each gene, and also contains 1000 control gRNAs for non targeted genomes. Among them, library A has designed four gRNAs for each miRNA. The two sub-libraries can be used separately or together.

Deliverable: 100ug plasmid (virus packaging-ready) QC: Coverage rate > 99%, uniformity < 10 Product details and prices: Request a quote>>

Applications of CRISPR Library

Screening functional genes of tumor

To screen the genes necessary for tumor growth and find new targets for tumor therapy.

To screen gene pairs with synthetic lethality for tumor therapy

To screen the genes causing drug resistance of tumors and study the mechanism of drug resistance

Viral infection related study

To screen host factors related to viral infection, including receptor and endoplasmic reticulum function related genes

Antibody target screening

Use CPRIPR library to validate the specificity of antibody

Signal pathway

Use CPRIPR library to study gene function and its mechanism in signal pathway

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