AACS Knockout cell line (U-2 OS) – Genotype Verified & Ready-to-use

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AACS Knockout cell line (U-2 OS)

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Catalog#

YKO-HB70997

Species

Human

Target Cell Line

U-2 OS

Size

1*10^6

Gene

AACS

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

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Overview

Gene knockout cell lines are generated using CRISPR-Cas9 based technology to precisely disrupt target genes, enabling functional genomics research, disease modeling, and target validation.

AACS (acetoacetyl-CoA synthetase) encodes an enzyme predicted to catalyze the formation of acetoacetyl-CoA from acetoacetate. AACS participates in cytosolic acetoacetate utilization and is predicted to contribute to metabolic regulation, including positive regulation of insulin secretion. It is therefore relevant to studies of ketone body metabolism and cellular metabolic signaling.

The U-2 OS cell line is a foundational human osteosarcoma model established in 1964 by J. Ponten and E. Saksela from a moderately differentiated osteogenic sarcoma of the tibia of a 15-year-old girl. Originally designated as 2T and referenced in image_2f37aa.png, this adherent epithelial-like model is documented to secrete osteosarcoma-derived growth factor (ODGF) and express functional insulin-like growth factor (IGF-1) receptors, insulin-like growth factor-II/Mannose-6-phosphate (IGF-II/M6P) receptors, and IGF-II. Serving as a gold-standard in vitro platform, it is extensively utilized in biomedical research for studying bone oncology pathways, cellular response kinetics, and advanced transfection signaling mechanisms.

Before delivery, each clone is authenticated by STR, tested for sterility and mycoplasma, and genotype‑verified by two‑round PCR‑sequencing. Comprehensive QC data accompany every shipment to support reproducible research.

KO Cell Construction Strategy

Adopting Red Cotton CRISPR Gene Editing Designer (www.rc-crispr.com), two sgRNAs targeting the AACS gene are designed to generate a knockout strategy. The two sgRNA sequences are subsequently cloned into the EZ-editor™ vector and introduced into U-2 OS cells via electroporation or lentiviral transduction. Single-cell clones are then generated using the limiting dilution method. Genomic DNA from individual clones is subjected to nucleic acid lysis and PCR amplification using the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000). The edited loci are further verified by Sanger sequencing to confirm the genotype. After secondary validation and quality confirmation, AACS Knockout cell line (U-2 OS) is expanded and cryopreserved for downstream applications.

Product Overview

AACS Knockout cell line (U-2 OS)

The AACS Knockout cell line (U-2 OS) is generated by targeted disruption of the AACS gene in U-2 OS cells. This cell line is designed to investigate the role of AACS in cellular function and gene regulation, as well as its potential involvement in disease-related pathways.

KO Cell Line

AACS Knockout cell line (U-2 OS)

Gene ID

65985

Catalog

YKO-HB70997

Gene Name

AACS

Product Category

Knockout(KO) Cell Line

Gene Information

NCBI

Species

Human

Mycoplasma test

Negative

Cell Morphology

Epithelial-like, adherent

Catalog Status

-

Culture method

90% McCOY's 5A+10%FBS

Shipping

Cryopreserved Cells shipped in dry ice

Passage ratio

1:3~1:5

Storage Condition

Liquid nitrogen

Quality Control

All KO cell products are tested and confirmed to be free of bacterial, viral, mycoplasma contamination, and other adventitious agents.

Applications

For research use only. Not intended for use in diagnostic, therapeutic, clinical, or other applications.

Key Advantages

Easy to use

Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations

High-efficient KO

These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.

Curated cells

Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.

Quality Control Specifications

1. Bacteria and Fungi Test

None Detected.

2. Mycoplasma test

Negative.

3. Knockout Strategy

Targeted knockout region: EExon 1

Reference Transcript: NM_023928

Frameshift Mutation Strategy

4. STR Authentication

Download STR Report

Intended use

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use

Unpacking and Storage Instructions

1.

Cell Reception

2.

Unpacking and storage instructions

Handling procedure

1.

Cell Thawing

2.

Cell Passaging

3.

Cell cryopreservation

References

More

FAQ

Q1. How to Contact Support if Issues Arise After Receiving the KO Cells?

If any abnormalities are observed after receiving the cells, please provide the following information based on the stage of use and contact us promptly for assistance:

(1) Issues upon delivery: Please provide photos of the cells at the time of receipt along with a brief description of the issue. We will offer after-sales support accordingly.

(2) Issues after thawing or passaging: Please provide microscopic images of the cells before and after thawing/passaging, along with a detailed description of the experimental procedures. Our technical team will provide professional support.

Q2. What Information Is Required When Reporting KO Cell Product Issues?

Q3. How Should the KO cell Product Be Stored Upon Receipt?

Q4. Shipping Method

Q5. Are the Knockout Cell Lines Stable for Passaging? Is Authentication Provided?