AADACL2 Knockout cell line (HK-2)

Ubigene gene knockout cell lines mediated by optimized CRISPR-Cas9 technology provide reliable in vitro models for investigating gene function, signaling pathways, disease mechanisms, and therapeutic targets.

HK-2 (Human Kidney-2) is a well-characterized immortalized human kidney proximal tubular epithelial cell line derived from normal adult renal tissue. Transformed via the transduction of human papillomavirus type 16 (HPV-16) E6/E7 genes, HK-2 successfully achieves continuous in vitro proliferation while stably retaining the functional and phenotypic characteristics of primary proximal tubular epithelium. HK-2 cells maintain distinct brush border enzyme activities and exhibit physiologic responsiveness to cytokines and nephrotoxic compounds. Consequently, HK-2 serves as a globally accepted, standard human cell platform extensively utilized in in vitro nephrotoxicity screening, renal fibrosis modeling, tubular transport mechanism profiling, and preclinical drug safety evaluations.

Comprehensive quality control includes STR authentication, sterility testing (bacteria and fungi), and mycoplasma screening to ensure purity and performance. Genotype is confirmed by two rounds of PCR and Sanger sequencing, and complete validation reports are provided for make-to-order products.