ABCB8 Knockout cell line (HCT-15)

Gene knockout cell lines are generated using CRISPR-Cas9 based technology to precisely disrupt target genes, enabling functional genomics research, disease modeling, and target validation.

ABCB8 (ATP binding cassette subfamily B member 8) encodes an ATP-dependent transporter localized to the mitochondrial inner membrane. ABCB8 is predicted to transport organic or inorganic molecules across the mitochondrial membrane and may contribute to mitochondrial metabolite and ion homeostasis. Its activity has also been linked to regulation of iron balance between mitochondria and the cytosol.

HCT-15 is a human colorectal adenocarcinoma cell line established from a colorectal tumor. The cells are tumorigenic in nude mice and share a common genetic origin with several colorectal cancer cell lines, including DLD-1, HCT-8, and HRT-18, as confirmed by STR profiling. HCT-15 is widely used as a model for studying colorectal cancer biology, tumorigenesis, genetic alterations, and anticancer drug responses.

Comprehensive quality control includes STR authentication, sterility testing (bacteria and fungi), and mycoplasma screening to ensure purity and performance. Genotype is confirmed by two rounds of PCR and Sanger sequencing, and complete validation reports are provided for make-to-order products.