HDAC6 knockout cell line (HEK293)

Gene knockout cell lines are generated using CRISPR-Cas9 based technology to precisely disrupt target genes, enabling functional genomics research, disease modeling, and target validation.

HDAC6 (histone deacetylase 6) encodes a class II histone deacetylase with two catalytic domains. It possesses histone deacetylase activity and contributes to transcriptional repression through regulation of protein acetylation and chromatin-associated processes.

The HEK293 cell line is a human embryonic kidney cell line derived from adenovirus 5-transformed human embryonic kidney cells. The cells express adenovirus 5 early region 1 (E1) antigens and are permissive for adenovirus 5 host range mutants defective in E1 functions. HEK293 cells have been adapted into various derivatives, including suspension-adapted variants and engineered reporter lines for specific applications. This cell line is widely used in molecular biology, viral vector production, recombinant protein expression, gene expression studies, and research involving cellular signaling and genome engineering.

Each knockout cell line is validated by STR authentication, sterility testing (bacteria/fungi), and mycoplasma screening. Genotype is confirmed by two rounds of PCR and Sanger sequencing. Only clones that pass all QC criteria are released, ensuring reliable identity and performance for downstream applications.