MFAP3 Knockout cell line (T24)

Gene knockout cell lines are generated using CRISPR-Cas9 based technology to precisely disrupt target genes, enabling functional genomics research, disease modeling, and target validation.

MFAP3 (microfibril associated protein 3) encodes a protein predicted to be localized to the extracellular region. The available information provides limited functional characterization of MFAP3, with its primary annotation supporting an extracellular localization. Its association with the extracellular environment suggests potential involvement in extracellular matrix-related processes, although specific molecular functions and cellular pathways are not sufficiently established by the provided information.

The T24 cell line was established in 1970 from a primary grade III transitional cell carcinoma (urothelial carcinoma) of the urinary bladder from an 81-year-old female patient. This human bladder carcinoma cell line is widely characterized and has been reported to carry HRAS and p53 mutations. T24 cells produce multiple cytokines, including granulocyte colony-stimulating factor (G-CSF), interleukin-6 (IL-6), and stem cell factor (SCF). The line is commonly used as a model for studying bladder cancer biology, oncogenic signaling pathways, cytokine regulation, and therapeutic responses in urothelial carcinoma.

Before delivery, each clone is authenticated by STR, tested for sterility and mycoplasma, and genotype‑verified by two‑round PCR‑sequencing. Comprehensive QC data accompany every shipment to support reproducible research.