Slc2a4 Knockout cell line (NIH/3T3)

CRISPR knockout cell lines are engineered through targeted genome editing to eliminate gene function, supporting studies of cellular biology, disease mechanisms, and drug discovery.

Established from a NIH Swiss mouse embryo, this widely selected cell line is highly sensitive to sarcoma virus focus formation and leukemia virus propagation. Notably, the current stock of this line has lost its natural contact inhibition—the physiological process where cell growth arrests upon cell-to-cell contact—and the broader impact of this loss on other cellular characteristics remains uncertain. Consequently, when choosing this popular line, researchers must critically evaluate whether contact inhibition is required for their specific application; it remains a vital prerequisite for assays measuring the expression or induction of potential oncogenes, whereas it carries significantly less weight when deploying the cell line for recombinant protein production and purification.

Before delivery, each clone is authenticated by STR, tested for sterility and mycoplasma, and genotype‑verified by two‑round PCR‑sequencing. Comprehensive QC data accompany every shipment to support reproducible research.