TBC1D31 Knockout cell line (ARPE-19)

CRISPR knockout cell lines are engineered through targeted genome editing to eliminate gene function, supporting studies of cellular biology, disease mechanisms, and drug discovery.

TBC1D31 (TBC1 domain family member 31) encodes a protein with molecular adaptor activity that is involved in cilium assembly. It localizes to the Golgi apparatus, centriolar satellites, and ciliary basal body, positioning it within cellular structures that coordinate centrosomal and ciliary organization. TBC1D31 therefore contributes to assembly and maintenance of primary ciliary structures.

The ARPE-19 cell line is a spontaneously arising human retinal pigment epithelial (RPE) cell line established in 1986 by Amy Aotaki-Keen from the normal eyes of a 19-year-old male donor. The cells exhibit characteristic RPE morphology, including the ability to form cobblestone-like monolayers and develop pigmentation during long-term culture. ARPE-19 provides a widely used in vitro model for studying retinal pigment epithelium biology, retinal function, ocular disease mechanisms, and cellular responses relevant to vision research.

Before delivery, each clone is authenticated by STR, tested for sterility and mycoplasma, and genotype‑verified by two‑round PCR‑sequencing. Comprehensive QC data accompany every shipment to support reproducible research.