Caco-2-CAS9 cell line

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location: Home > Products > Cas9 Stable Cell Lines > Caco-2-CAS9
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  • Caco-2 Caco-2-CAS9-Caco-2
  • Caco-2-CAS9 cell line Caco-2-CAS9-产品图
Caco-2-CAS9 Copy Product Info
Catalog#: YC-D003-Cas9-H
Size: 1*10^6
Culture method: 77%MEM+20%FBS+1%Glutamax+1%Sodium Pyruvate+1%NEAA
  • Caco-2 Caco-2-CAS9-Caco-2
  • Caco-2-CAS9 cell line Caco-2-CAS9-产品图
Caco-2-CAS9 Copy Product Info
Catalog#: YC-D003-Cas9-H
Size: 1*10^6
Culture method: 77%MEM+20%FBS+1%Glutamax+1%Sodium Pyruvate+1%NEAA

Detailed Product Information

Cell Info

Product category Cas9 Stable Cell Lines Mycoplasma test negative
Cell Morphology Epithelial-like, adherent Culture method 77%MEM+20%FBS+1%Glutamax+1%Sodium Pyruvate+1%NEAA
Passage ratio 1:2~1:4 Species Human
Expression gene Cas9 Resistance gene hygro
Product category
Cas9 Stable Cell Lines
Mycoplasma test
negative
Cell Morphology
Epithelial-like, adherent
Culture method
77%MEM+20%FBS+1%Glutamax+1%Sodium Pyruvate+1%NEAA
Passage ratio
1:2~1:4
Species
Human
Expression gene
Cas9
Resistance gene
hygro
Recombinant Cas9 protein derived from Streptococcus pyogenes is a protein that plays a key role in CRISPR/Cas9 system. It has endonuclease activity; Under the guidance of sgRNA, Cas9 protein can move to the target gene site, cut DNA accurately, and cause DNA double-strand breaks. Ubigene's Caco-2 cell line was constructed on the basis of wild-type Caco-2 cells by lentiviral transduction of the engineered Cas9 protein gene, which can stably express Cas9 protein for a long time. Using this cell, efficient gene knockout, knock-in and point mutation in Caco-2 cells can be achieved by only transfecting sgRNA or co-transfecting sgRNA and donor DNA. It can also be used for the construction of cell knockout library and easily solve the problem of Cas9 transfection difficulty.

Product Validation Data

RT-qPCR
Sample Name Target Name Cт Mean ΔCт
Caco-2-CAS9 Cas9 19.79857635 3.43322181
Caco-2-CAS9 β-actin 16.36535454
Caco-2 Cas9 36.82312775 19.86952782
Caco-2 β-actin 16.95359993
Cutting Efficiency Validation
Caco-2-CAS9 cell line Product Validation Data(RT-QPCR)
Note: The above figure shows the sequencing peaks of the Caco-2-CAS9 stable cell pool which is electroporated by TAF15 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:Caco-2
STR Info (Cell bank)
Cell Line:CACO-2
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12131213
D13S317 1114111413.0
D7S820 11121112
D16S539 12121213
VWA 16181618
TH01 6666
AMEL XXXX
TPOX 911911
CSF1PO 11111111
D12S391 1723
FGA 1919
D2S1338 1725
D21S11 3030
D18S51 1212
D8S1179 1212
D3S1358 1717
D6S1043 1212
PENTAE 77
D19S433 1515
PENTAD 99
D1S1656 1516
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

  • icon
    Easy to use
    Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
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    High-efficient KO
    These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
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    Curated cells
    Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.

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