HMC3-CAS9 cell line

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  • Wild-type Cell Lines- HMC3-CAS9-Wild-type Cell Lines-
  • HMC3-CAS9 cell line HMC3-CAS9-产品图
Wild-type Cell Lines- HMC3-CAS9-Wild-type Cell Lines-
HMC3-CAS9 cell line HMC3-CAS9-产品图
HMC3-CAS9 Copy Product Info
Catalog#： YC-C131-Cas9-H
Size： 1*10^6
Culture method： 89%MEM+10%FBS+1% NEAA
STR Report
Datasheet (Use Instruction)
  • Wild-type Cell Lines- HMC3-CAS9-Wild-type Cell Lines-
  • HMC3-CAS9 cell line HMC3-CAS9-产品图
HMC3-CAS9 Copy Product Info
Catalog#： YC-C131-Cas9-H
Size： 1*10^6
Culture method： 89%MEM+10%FBS+1% NEAA
Datasheet (Use Instruction)

Detailed Product Information

Cell Info

Product category Cas9 Stable Cell Lines Mycoplasma test negative
Cell Morphology Epithelial-like, adherent Culture method 89%MEM+10%FBS+1% NEAA
Passage ratio 1：2~1：3 Species Human
Expression gene Cas9 Resistance gene hygro
Product category
Cas9 Stable Cell Lines
Mycoplasma test
negative
Cell Morphology
Epithelial-like, adherent
Culture method
89%MEM+10%FBS+1% NEAA
Passage ratio
1：2~1：3
Species
Human
Expression gene
Cas9
Resistance gene
hygro
Recombinant Cas9 protein derived from Streptococcus pyogenes is a protein that plays a key role in CRISPR/Cas9 system. It has endonuclease activity; Under the guidance of sgRNA, Cas9 protein can move to the target gene site, cut DNA accurately, and cause DNA double-strand breaks. Ubigene's HMC3 cell line was constructed on the basis of wild-type HMC3 cells by lentiviral transduction of the engineered Cas9 protein gene, which can stably express Cas9 protein for a long time. Using this cell, efficient gene knockout, knock-in and point mutation in HMC3 cells can be achieved by only transfecting sgRNA or co-transfecting sgRNA and donor DNA. It can also be used for the construction of cell knockout library and easily solve the problem of Cas9 transfection difficulty.

Product Validation Data

RT-qPCR
Sample Name Target Name Cт Mean ΔCт
HMC3-CAS9 Cas9 21.16933632 2.45841599
HMC3-CAS9 β-actin 18.71092033
HMC3 Cas9 31.39811516 14.71577072
HMC3 β-actin 16.68234444
Cutting Efficiency Validation
HMC3-CAS9 cell line Product Validation Data（RT-QPCR）
Note: The above figure shows the sequencing peaks of the HMC3-CAS9 stable cell pool which is electroporated by CLEC3B gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.
STR Profile
Download STR Report
Loci STR Info (Sample Cell)
Sample Cell Line:HMC3		 STR Info (Cell bank)
Cell Line:HMC3
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 11111111
D7S820 911911
D16S539 12131213
VWA 17191719
TH01 6666
AMEL XXXX
TPOX 8989
CSF1PO 10111011
D12S391 1621
FGA 2125
D2S1338 1725
D21S11 3031.2
D18S51 1818
D8S1179 1314
D3S1358 1618
D6S1043 1111
PENTAE 713
D19S433 1515.2
PENTAD 1014
D1S1656 1213
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

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    Easy to use
    Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
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    High-efficient KO
    These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
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    Curated cells
    Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
Related Resources: Red Cotton Gene-editing System | ATCC | DSMZ | ECAZZ | Cellosaurus | CCTCC | NCBI
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