Jurkat, Clone E6-1-CAS9 cell line

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location: Home > Products > Cas9 Stable Cell Lines > Jurkat, Clone E6-1-CAS9
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  • Jurkat, Clone E6-1 Jurkat, Clone E6-1-CAS9-Jurkat, Clone E6-1
  • Jurkat, Clone E6-1-CAS9 cell line Jurkat, Clone E6-1-CAS9-产品图
Jurkat, Clone E6-1-CAS9 Copy Product Info
Catalog#: YC-D012-Cas9-H
Size: 1*10^6
Culture method: 90%RPMI-1640+10%FBS
  • Jurkat, Clone E6-1 Jurkat, Clone E6-1-CAS9-Jurkat, Clone E6-1
  • Jurkat, Clone E6-1-CAS9 cell line Jurkat, Clone E6-1-CAS9-产品图
Jurkat, Clone E6-1-CAS9 Copy Product Info
Catalog#: YC-D012-Cas9-H
Size: 1*10^6
Culture method: 90%RPMI-1640+10%FBS

Detailed Product Information

Cell Info

Product category Cas9 Stable Cell Lines Mycoplasma test negative
Cell Morphology Lymphocyte-like,suspension Culture method 90%RPMI-1640+10%FBS
Passage ratio 1:3-1:5 Species Human
Expression gene Cas9 Resistance gene hygro
Product category
Cas9 Stable Cell Lines
Mycoplasma test
negative
Cell Morphology
Lymphocyte-like,suspension
Culture method
90%RPMI-1640+10%FBS
Passage ratio
1:3-1:5
Species
Human
Expression gene
Cas9
Resistance gene
hygro
Recombinant Cas9 protein derived from Streptococcus pyogenes is a protein that plays a key role in CRISPR/Cas9 system. It has endonuclease activity; Under the guidance of sgRNA, Cas9 protein can move to the target gene site, cut DNA accurately, and cause DNA double-strand breaks. Ubigene's Jurkat, Clone E6-1 cell line was constructed on the basis of wild-type Jurkat, Clone E6-1 cells by lentiviral transduction of the engineered Cas9 protein gene, which can stably express Cas9 protein for a long time. Using this cell, efficient gene knockout, knock-in and point mutation in Jurkat, Clone E6-1 cells can be achieved by only transfecting sgRNA or co-transfecting sgRNA and donor DNA. It can also be used for the construction of cell knockout library and easily solve the problem of Cas9 transfection difficulty.

Product Validation Data

RT-qPCR
Sample Name Target Name Cт Mean ΔCт
Jurkat, Clone E6-1-CAS9 Cas9 17.92595673 1.44584656
Jurkat, Clone E6-1-CAS9 β-actin 16.48011017
Jurkat, Clone E6-1 Cas9 31.83985138 13.98332977
Jurkat, Clone E6-1 β-actin 17.85652161
Cutting Efficiency Validation
Jurkat, Clone E6-1-CAS9 cell line Product Validation Data(RT-QPCR)
Note: The above figure shows the sequencing peaks of the Jurkat, Clone E6-1-CAS9 stable cell pool which is electroporated by DCAF11 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:Jurkat, Clone E6-1
STR Info (Cell bank)
Cell Line:Jurkat, Clone E6-1
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 9999
D13S317 812812
D7S820 812812
D16S539 11111111
VWA 18181818
TH01 69.369.3
AMEL XYXY
TPOX 810810
CSF1PO 11121112
D12S391 2223
FGA 2021
D2S1338 1923
D21S11 31.233.2
D18S51 132122.0
D8S1179 1314
D3S1358 1515
D6S1043 1111
PENTAE 1012
D19S433 1415.2
PENTAD 1113
D1S1656 1617
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

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    Easy to use
    Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
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    High-efficient KO
    These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
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    Curated cells
    Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.

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