Jurkat, Clone E6-1-CAS9

location: Home > Products > Cas9 Stable Cell Lines > Jurkat, Clone E6-1-CAS9
All products
  • All products
  • Red Cotton™ gRNA Plasmid Bank
  • KO Cell Lines
  • Wild-type Cell Lines
  • Cas9 Stable Cell Lines
  • Luciferase Stable Cell Lines
  • EGFP Stable Cell Lines
  • Stem Cell Lines
  • Transfection Culture Medium
  • Cell Monoclonal Culture Medium
  • EZ-Stem™ Cell Culture Medium
  • EZ-cryo™ cell freezing medium
  • Kits
  • Lentivirus
Jurkat, Clone E6-1-CAS9

Jurkat, Clone E6-1-CAS9 Order


Price (USD)-

Size 1*10^6


Jurkat, Clone E6-1 Cell Line was generated by CRISPR-U™ gene-editing technology to stably express Cas9 protein.

Cell Info

Catalog YC-D012-Cas9-H
Cell line Jurkat, Clone E6-1-CAS9 Cell Type Human T lymphocyte cell line
Morphology Lymphocyte-like, suspension Passage ratio Control the cell density at 2x10^5~4.0x10^5 cells/ml during passaging, and carry out the passage when the cells grow to 8x10^5~1.0x10^6 cells/ml.
Culture method 90%RPMI-1640+10%FBS
Cryopreservation solution 50%RPMI-1640+40%FBS+10%DMSO
Special Note The cell culture density needs to be strictly controlled.

Product Validation Data

Sample NameTarget NameCт MeanΔCт
Jurkat, Clone E6-1-CAS9Cas917.925956731.44584656
Jurkat, Clone E6-1-CAS9h-actin-B16.48011017
Jurkat, Clone E6-1Cas931.8398513813.98332977
Jurkat, Clone E6-1h-actin-B17.85652161
Cutting Efficiency Validation
Note: The above figure shows the sequencing peaks of the Jurkat, Clone E6-1-CAS9 stable cell pool which is electroporated by CHD4 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.

Product Advantage

  • (1)Easy to use:Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
  • (2)High-efficient KO:These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
  • (3)High-quality cell:Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.


Jurkat, Clone E6-1

Contact us