Jurkat, Clone E6-1-CAS9

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Jurkat, Clone E6-1-CAS9 Order

Catalog#：YC-D012-Cas9-H

Price (USD)：-

Size： 1*10^6

Instruction：

Jurkat, Clone E6-1 Cell Line was generated by CRISPR-U™ gene-editing technology to stably express Cas9 protein.

Detailed Product Information Recommended Companion Products

Cell Info

Catalog	YC-D012-Cas9-H
Cell line	Jurkat, Clone E6-1-CAS9	Cell Type	Human T lymphocyte cell line
Morphology	Lymphocyte-like, suspension	Passage ratio	Control the cell density at 2x10^5~4.0x10^5 cells/ml during passaging, and carry out the passage when the cells grow to 8x10^5~1.0x10^6 cells/ml.
Culture method	90%RPMI-1640+10%FBS
Cryopreservation solution	50%RPMI-1640+40%FBS+10%DMSO
Special Note	The cell culture density needs to be strictly controlled.

Product Validation Data

RT-QPCR

Sample Name	Target Name	Cт Mean	ΔCт
Jurkat, Clone E6-1-CAS9	Cas9	17.92595673	1.44584656
Jurkat, Clone E6-1-CAS9	h-actin-B	16.48011017	1.44584656
Jurkat, Clone E6-1	Cas9	31.83985138	13.98332977
Jurkat, Clone E6-1	h-actin-B	17.85652161	13.98332977

Cutting Efficiency Validation

Note: The above figure shows the sequencing peaks of the Jurkat, Clone E6-1-CAS9 stable cell pool which is electroporated by CHD4 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.

Product Advantage

（1）Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
（2）High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
（3）High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.