KYSE-150-CAS9 cell line

×

Subscribe Us

By subscription, you consent to allow Ubigene Biosciences to store and process the information provided above to deliver the latest news, research spotlight, and promotions. You can unsubscribe from these communications at anytime.
location: Home > Products > Cas9 Stable Cell Lines > KYSE-150-CAS9
All products
  • All products
  • Red Cotton™ gRNA Plasmid Bank
  • KO Cell Lines
  • Wild-type Cell Lines
  • Cas9 Stable Cell Lines
  • Luciferase Stable Cell Lines
  • EGFP Stable Cell Lines
  • Stem Cell Lines
  • Transfection Culture Medium
  • Cell Monoclonal Culture Medium
  • EZ-Stem™ Cell Culture Medium
  • CRISPR-iScreen™ Library Plasmid
  • CRISPR-iScreen™ Library Virus
  • CRISPR-iScreen™ Cell Pool
  • EZ-cryo™ cell freezing medium
  • Kits
  • Lentivirus
  • KYSE-150 KYSE-150-CAS9-KYSE-150
  • KYSE-150-CAS9 cell line KYSE-150-CAS9-产品图
KYSE-150-CAS9 Copy Product Info
Catalog#: YC-B002-Cas9-H
Size: 1*10^6
Culture method: 90%RPMI-1640+10%FBS
  • KYSE-150 KYSE-150-CAS9-KYSE-150
  • KYSE-150-CAS9 cell line KYSE-150-CAS9-产品图
KYSE-150-CAS9 Copy Product Info
Catalog#: YC-B002-Cas9-H
Size: 1*10^6
Culture method: 90%RPMI-1640+10%FBS

Detailed Product Information

Cell Info

Product category Cas9 Stable Cell Lines Mycoplasma test negative
Cell Morphology Epithelial-like, adherent Culture method 90%RPMI-1640+10%FBS
Passage ratio 1:2~1:4 Species Human
Expression gene Cas9 Resistance gene hygro
Product category
Cas9 Stable Cell Lines
Mycoplasma test
negative
Cell Morphology
Epithelial-like, adherent
Culture method
90%RPMI-1640+10%FBS
Passage ratio
1:2~1:4
Species
Human
Expression gene
Cas9
Resistance gene
hygro
Recombinant Cas9 protein derived from Streptococcus pyogenes is a protein that plays a key role in CRISPR/Cas9 system. It has endonuclease activity; Under the guidance of sgRNA, Cas9 protein can move to the target gene site, cut DNA accurately, and cause DNA double-strand breaks. Ubigene's KYSE-150 cell line was constructed on the basis of wild-type KYSE-150 cells by lentiviral transduction of the engineered Cas9 protein gene, which can stably express Cas9 protein for a long time. Using this cell, efficient gene knockout, knock-in and point mutation in KYSE-150 cells can be achieved by only transfecting sgRNA or co-transfecting sgRNA and donor DNA. It can also be used for the construction of cell knockout library and easily solve the problem of Cas9 transfection difficulty.

Product Validation Data

RT-qPCR
Sample Name Target Name Cт Mean ΔCт
KYSE-150-CAS9 Cas9 14.41407394 -1.49167920
KYSE-150-CAS9 β-actin 15.90575314
KYSE-150 Cas9 36.77395630 20.31886864
KYSE-150 β-actin 16.45508766
Cutting Efficiency Validation
KYSE-150-CAS9 cell line Product Validation Data(RT-QPCR)
Note: The above figure shows the sequencing peaks of the KYSE-150-CAS9 stable cell pool which is electroporated by CD274 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:KYSE-150
STR Info (Cell bank)
Cell Line:KYSE-150
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12131213
D13S317 811811
D7S820 10111011
D16S539 911911
VWA 16171617
TH01 7979
AMEL XXXX
TPOX 8888
CSF1PO 12131213
D12S391 1922
FGA 2124
D2S1338 2525
D21S11 3031
D18S51 1414
D8S1179 1015
D3S1358 1516
D6S1043 1820
PENTAE 1218
D19S433 1515
PENTAD 1010
D1S1656 1517.3
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

  • icon
    Easy to use
    Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
  • icon
    High-efficient KO
    These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
  • icon
    Curated cells
    Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.

Related Popular Products

KO Cell Lines

  • SLC25A5 Knockout cell line (KYSE-150)

    SLC25A5 Knockout cell line (KYSE-150)

    Organism: Human
    Gene Name: SLC25A5
    Gene ID: 292
    Size: 1*10^6
    Catalog#: YKO-H138
    YKO-H138(KYSE-150)

Luciferase Stable Cell Lines

Wild-type Cell Lines

Related Popular Products

KO Cell Lines

  • SLC25A5 Knockout cell line (KYSE-150)

    SLC25A5 Knockout cell line (KYSE-150)

    Organism: Human
    Gene Name: SLC25A5
    Gene ID: 292
    Size: 1*10^6
    Catalog#: YKO-H138

Luciferase Stable Cell Lines

Wild-type Cell Lines

  • KYSE-150 cell line

    Human Esophageal Squamous Carcinoma Cell Line(KYSE-150)

    Price (USD): -
    Catalog#: YC-B002
    Size: 1*10^6
    Instruction: Human Esophageal Squamous Carcinoma Cell Line(KYSE-150)

×

Search documentation

Literature:

Name: *

Company: *

Telephone: *

Email:

Notes: After submitting the order, we will contact you as soon as possible.

request now
Contact us