MC3T3-E1 Subclone 14-CAS9 cell line

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location： Home > Products > Cas9 Stable Cell Lines > MC3T3-E1 Subclone 14-CAS9

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MC3T3-E1 Subclone 14-CAS9 Order

MC3T3-E1 Subclone 14-CAS9 cell line
Catalog#
YC-C026-Cas9-H
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Datasheet
MC3T3-E1 Subclone 14-CAS9 cell line
Order

Detailed Product Information

Product category
Cas9 Stable Cell Lines
cell name
MC3T3-E1 Subclone 14-CAS9
Morphology
Fibroblast-like, adherent
Passage ratio
1:3~1:5
Species
Mouse
Expression gene
Cas9
Resistance gene
hygro
Construction method
Lentivirus
Mycoplasma test
negative
Culture method
90% Alpha MEM+10%FBS
Description
Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the MC3T3-E1 Subclone 14 cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a MC3T3-E1 Subclone 14-CAS9 cell line that can maintain long-term, stable and high expression.
Product Validation Data（RT-QPCR）
Sample Name Target Name Cт Mean ΔCт
MC3T3-E1 Subclone 14-CAS9 Cas9 21.01457405 5.88625145
MC3T3-E1 Subclone 14-CAS9 h-actin-B 15.12832260
MC3T3-E1 Subclone 14 Cas9 33.41728210 18.85072040
MC3T3-E1 Subclone 14 h-actin-B 14.56656170
Cutting Efficiency Validation
MC3T3-E1 Subclone 14-CAS9 cell line Product Validation Data（RT-QPCR）
Note: The above figure shows the sequencing peaks of the MC3T3-E1 Subclone 14-CAS9 stable cell pool which is electroporated by Inpp5k gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:MC3T3-E1 Subclone 14		 STR Info (Cell bank)
Cell Line:MC3T3-E1 Subclone 14
Allele1Allele2Allele3Allele4Allele1Allele2Allele3
18-3 15.0
4-2 20.320.3
6-7 17.017.0
19-2 13.0
1-2 19.0
7-1 26.2
8-1 16.0
1-1 16.017.0
3-2 14.0
2-1 15.016.0
15-3 19.222.322.3
6-4 18.018.0
13-1 16.1
11-2 16.0
17-2 16.0
12-1 17.017.0
5-5 17.017.0
X-1 28.028.0
TH01
D5S818
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

  • Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
    01
  • High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
    02
  • High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
    03

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