MDA-MB-231-CAS9

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With 14 years of experience, Ubigene has exclusively innovated and developed 6 product lines, fullfilling all kinds of needs from researchers. Experiment process simplified, efficiency improved, achieving our aim of 'Make genome editing easier'!

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location： Home > Products > Cas9 Stable Cell Lines > MDA-MB-231-CAS9

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Cas9 Stable Cell Lines

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Brain and Nervous System

Circulatory System

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Urinary System

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Respiratory System

Endocrine System

Digestive System

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Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Brain and Nervous System

Circulatory System

Reproductive System

Urinary System

Blood and Lymphatic System

Respiratory System

Endocrine System

Digestive System

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Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Brain and Nervous System

Circulatory System

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Urinary System

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Respiratory System

Endocrine System

Digestive System

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Transfection Culture Medium

Cell Monoclonal Culture Medium

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MDA-MB-231-CAS9 Order

Catalog#：YC-D005-Cas9-H

Price (USD)：-

Size： 1*10^6

Instruction：

MDA-MB-231 Cell Line was generated by CRISPR-U™ gene-editing technology to stably express Cas9 protein.

Detailed Product Information Recommended Companion Products

Cell Info

Catalog	YC-D005-Cas9-H
Cell line	MDA-MB-231-CAS9	Cell Type	Human Breast Cancer Cell Line
Morphology	Epithelial-like, adherent	Passage ratio	1:3~1:4
Culture method	90%L-15+10%FBS
Cryopreservation solution	90%FBS+10%DMSO
Special Note	L-15 medium is formulated for growth of cells in CO2 free system. If using CO2 incubator, seal the flask cap tightly to ensure a CO2-free environment

Product Validation Data

RT-QPCR

Sample Name	Target Name	Cт Mean	ΔCт
MDA-MB-231-CAS9	Cas9	15.79268646	0.57719517
MDA-MB-231-CAS9	h-actin-B	15.21549129	0.57719517
MDA-MB-231	Cas9	31.13439941	15.70324325
MDA-MB-231	h-actin-B	15.43115616	15.70324325

Cutting Efficiency Validation

Note: The above figure shows the sequencing peaks of the MDA-MB-231-CAS9 stable cell pool which is electroporated by CHD4 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.

Product Advantage

（1）Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
（2）High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
（3）High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.