MDA-MB-231-CAS9 cell line

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MDA-MB-231
MDA-MB-231-CAS9 cell line

MDA-MB-231 MDA-MB-231-CAS9-MDA-MB-231

MDA-MB-231-CAS9 cell line MDA-MB-231-CAS9-产品图

MDA-MB-231-CAS9 Copy Product Info

Catalog#： YC-D005-Cas9-H

Size： 1*10^6

Culture method： 90%L-15+10%FBS(Recommend to use Ubigene-optimized culture medium)

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STR Report

Datasheet (Use Instruction)

MDA-MB-231
MDA-MB-231-CAS9 cell line

MDA-MB-231-CAS9 Copy Product Info

Catalog#： YC-D005-Cas9-H

Size： 1*10^6

Culture method： 90%L-15+10%FBS(Recommend to use Ubigene-optimized culture medium)

Order

STR Report

Datasheet (Use Instruction)

Detailed Product Information

Cell Info

Product category	Cas9 Stable Cell Lines	Mycoplasma test	negative
Cell Morphology	Epithelial-like, adherent	Culture method	90%L-15+10%FBS(Recommend to use Ubigene-optimized culture medium)
Passage ratio	1:2~1:4	Species	Human
Expression gene	Cas9	Resistance gene	hygro

Product category

Cas9 Stable Cell Lines

Mycoplasma test

negative

Cell Morphology

Epithelial-like, adherent

Culture method

90%L-15+10%FBS(Recommend to use Ubigene-optimized culture medium)

Passage ratio

1:2~1:4

Species

Human

Expression gene

Cas9

Resistance gene

hygro

Recombinant Cas9 protein derived from Streptococcus pyogenes is a protein that plays a key role in CRISPR/Cas9 system. It has endonuclease activity; Under the guidance of sgRNA, Cas9 protein can move to the target gene site, cut DNA accurately, and cause DNA double-strand breaks. Ubigene's MDA-MB-231 cell line was constructed on the basis of wild-type MDA-MB-231 cells by lentiviral transduction of the engineered Cas9 protein gene, which can stably express Cas9 protein for a long time. Using this cell, efficient gene knockout, knock-in and point mutation in MDA-MB-231 cells can be achieved by only transfecting sgRNA or co-transfecting sgRNA and donor DNA. It can also be used for the construction of cell knockout library and easily solve the problem of Cas9 transfection difficulty.

Product Validation Data

RT-qPCR

Sample Name	Target Name	Cт Mean	ΔCт
MDA-MB-231-CAS9	Cas9	15.79268646	0.57719517
MDA-MB-231-CAS9	β-actin	15.21549129	0.57719517
MDA-MB-231	Cas9	31.13439941	15.70324325
MDA-MB-231	β-actin	15.43115616	15.70324325

Cutting Efficiency Validation

MDA-MB-231-CAS9 cell line Product Validation Data（RT-QPCR）

Note: The above figure shows the sequencing peaks of the MDA-MB-231-CAS9 stable cell pool which is electroporated by NETO2 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.

STR Profile

Download STR Report

Loci	STR Info (Sample Cell) Sample Cell Line:MDA-MB-231			STR Info (Cell bank) Cell Line:MDA-MB-231
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	12	12		12	12
D13S317	13	13		13	13
D7S820	8	9		8	9
D16S539	12	12		12	12
VWA	15	18		15	18
TH01	7	9.3		7	9.3
AMEL	X	X		X	X
TPOX	8	9		8	9
CSF1PO	12	13		12	13
D12S391	17	18
FGA	22	23
D2S1338	20	21
D21S11	33.2	33.2
D18S51	11	16
D8S1179	13	13
D3S1358	16	16
D6S1043	18	18
PENTAE	11	11
D19S433	11	14
PENTAD	11	14
D1S1656	15	17

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

Easy to use

Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
High-efficient KO

These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
Curated cells

Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.

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MDA-MB-231-CAS9 cell line

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